Bonfield T L, Konstan M W, Burfeind P, Panuska J R, Hilliard J B, Berger M
Department of Pediatrics, Case Western Reserve University, Cleveland, Ohio, USA.
Am J Respir Cell Mol Biol. 1995 Sep;13(3):257-61. doi: 10.1165/ajrcmb.13.3.7544594.
Interleukin-10 (IL-10) is a potent regulatory cytokine that decreases inflammatory responses and T-cell stimulation. We have found that respiratory epithelial lining fluid (ELF) from patients with cystic fibrosis (CF) contains significantly less soluble IL-10 than ELF of healthy control subjects. Although macrophages from the chronically infected lungs of CF patients appear to be one source of IL-10, little or no intracellular IL-10 was found in bronchoalveolar lavage macrophages from healthy control subjects, suggesting that there must be another source of this cytokine in healthy lungs. We found that bronchial epithelial cells from healthy control subjects constitutively produce IL-10, which appears to be downregulated in CF patients. It is thus likely that the bronchial epithelium plays an important role in regulating the local immune response, producing IL-10 to decrease inflammation in the healthy lung. Conversely, downregulation of epithelial IL-10 production in CF airways may contribute to enhancing local inflammation and tissue damage.
白细胞介素-10（IL-10）是一种强效调节性细胞因子，可降低炎症反应和T细胞刺激。我们发现，囊性纤维化（CF）患者的呼吸道上皮衬液（ELF）中可溶性IL-10的含量明显低于健康对照受试者的ELF。尽管CF患者慢性感染肺部的巨噬细胞似乎是IL-10的一个来源，但在健康对照受试者的支气管肺泡灌洗巨噬细胞中几乎未发现细胞内IL-10，这表明健康肺部中一定存在这种细胞因子的另一个来源。我们发现，健康对照受试者的支气管上皮细胞组成性产生IL-10，而在CF患者中这种产生似乎下调。因此，支气管上皮很可能在调节局部免疫反应中起重要作用，通过产生IL-10来减轻健康肺部的炎症。相反，CF气道中上皮IL-10产生的下调可能有助于增强局部炎症和组织损伤。
