Leigh I M, Purkis P E, Whitehead P, Lane E B
ICRF Skin Tumour Laboratory, London Hospital Medical College, U.K.
Br J Dermatol. 1993 Aug;129(2):110-9. doi: 10.1111/j.1365-2133.1993.tb03511.x.
Monospecific antibodies to individual keratin polypeptides can be used to examine the tissue and cellular coexpression of members of keratin pairs. Monospecific monoclonal and polyclonal antibodies have been raised to keratins 1 and 10 using both crude cytoskeletal extracts and synthetic peptides. The tissue distribution of these keratins has been determined against a panel of freshly frozen normal tissues from humans, rodents and pigs. Epidermal expression has been examined in psoriatic plaques, and healing wounds, as examples of epidermal hyperproliferation. Cultured keratinocytes in monolayer (low calcium), stratified (high calcium), and complex cultures, transformed keratinocytes, and tumour cell lines, have been examined for the in vitro expression of these keratins. The sensitivity and precise localization of reactivity with these monospecific antibodies gives a highly accurate picture of individual cell expression. There is confirmation of coexpression of keratins 1 and 10 in epidermal and mucosal sites, and with keratin 16 in hyperproliferative states. These monospecific antibodies provide an important means of examining keratin expression in epidermal tumours and keratinizing disorders, and of seeking keratin mutations in cell lines and in skin diseases.
针对单个角蛋白多肽的单特异性抗体可用于检测角蛋白对成员在组织和细胞中的共表达情况。利用粗制细胞骨架提取物和合成肽已制备出针对角蛋白1和角蛋白10的单特异性单克隆抗体和多克隆抗体。已针对一组来自人类、啮齿动物和猪的新鲜冷冻正常组织确定了这些角蛋白的组织分布。作为表皮过度增殖的实例，已在银屑病斑块和愈合伤口中检测了表皮表达情况。已检测了单层（低钙）、分层（高钙）和复杂培养体系中的培养角质形成细胞、转化角质形成细胞以及肿瘤细胞系中这些角蛋白的体外表达情况。这些单特异性抗体反应的敏感性和精确定位给出了单个细胞表达的高度准确图景。已证实角蛋白1和角蛋白10在表皮和黏膜部位共表达，且在增殖状态下与角蛋白16共表达。这些单特异性抗体为检测表皮肿瘤和角化异常中的角蛋白表达以及在细胞系和皮肤病中寻找角蛋白突变提供了重要手段。
