Blottière H M, Daculsi G, Anegon I, Pouezat J A, Nelson P N, Passuti N
Laboratoire de Recherche sur les Matériaux d'Intérêt Biologique, Faculté de Chirurgie Dentaire, Nantes, France.
Biomaterials. 1995 Apr;16(6):497-503. doi: 10.1016/0142-9612(95)98824-x.
The use of calcium phosphate biomaterials as a bone substitute necessitates the use of normative biocompatibility and biodegradation techniques which must be fast, simple and reproducible. In the present study, we have developed an in vitro model to study and to compare different calcium phosphate ceramics. After activation with 1,25-dihydroxy-vitamin D3 and phorbol 12,13-dibutyrate, the monoblastic U937 cells became multinucleated, expressed tartrate-resistant acid phosphatase and several markers of monocyte/macrophage differentiation. Activated U937 cells did not express the vitronectin receptor (VNR) (as revealed using monoclonal antibodies 23C6 or 13C2) but around 25% of the cells were strongly reactive with 211D, a novel monoclonal antibody that recognizes an osteoclast-specific membrane antigenic determinant. These cells remain active/viable with hydroxyapatite (HA) or beta-tricalcium phosphate (beta-TCP) ceramics. In conclusion, activated U937 cells are good candidates to use in a normative in vitro method to evaluate new biomaterials.
将磷酸钙生物材料用作骨替代物需要使用规范的生物相容性和生物降解技术，这些技术必须快速、简便且可重复。在本研究中，我们开发了一种体外模型来研究和比较不同的磷酸钙陶瓷。在用1,25 - 二羟基维生素D3和佛波醇12,13 - 二丁酸酯激活后，单核细胞样U937细胞变成多核细胞，表达抗酒石酸酸性磷酸酶以及单核细胞/巨噬细胞分化的几种标志物。激活的U937细胞不表达玻连蛋白受体（VNR）（如使用单克隆抗体23C6或13C2所显示），但约25%的细胞与211D强烈反应，211D是一种识别破骨细胞特异性膜抗原决定簇的新型单克隆抗体。这些细胞在羟基磷灰石（HA）或β - 磷酸三钙（β - TCP）陶瓷上仍保持活性/存活。总之，激活的U937细胞是用于规范体外方法评估新型生物材料的良好候选者。
