Oomori Y, Satoh Y, Ishikawa K, Ono K
Department of Anatomy, Asahikawa Medical College, Japan.
Histochem J. 1995 May;27(5):395-400.
The present immunohistochemical study revealed substance P-immunoreactive neuronal elements in the von Ebner's gland of rats. Immunoreactive ganglion cells were observed as single cells or groups of several immunoreactive ganglion cells among intra-lingual muscles, at the base of the vallate papillae and near the von Ebner's gland. Very numerous substance P-immunoreactive varicose nerve fibres ran closely associated with the serous cells and excretory duct cells, and were seen to run along blood vessels in the gland. Since substance P-immunoreactive ganglion cells were present near the glands, the immunoreactive varicose nerve fibres in the von Ebner's gland may be partly derived from the intra-lingual ganglion cells. These substance P-immunoreactive varicose nerve fibres may have an effect on the secretory activity of the serous cells and duct cells, and on the vasodilation of blood vessels of the von Ebner's gland. Actin immunoreactivity was seen in numerous myoepithelial cells embracing serous cells and duct cells, and in the smooth muscle cells of blood vessels of the gland. By using a double immunolabelling technique with anti-substance P and anti-actin sera, substance P-immunoreactive varicose nerve fibres were found to be in close contact with myoepithelial cells.
目前的免疫组织化学研究揭示了大鼠味腺中P物质免疫反应性神经元成分。在舌内肌之间、轮廓乳头基部和味腺附近，免疫反应性神经节细胞以单个细胞或几个免疫反应性神经节细胞的群体形式被观察到。大量P物质免疫反应性曲张神经纤维与浆液细胞和排泄管细胞紧密相连，并可见其沿腺体内的血管走行。由于在腺体附近存在P物质免疫反应性神经节细胞，味腺中的免疫反应性曲张神经纤维可能部分源自舌内神经节细胞。这些P物质免疫反应性曲张神经纤维可能对浆液细胞和导管细胞的分泌活动以及味腺血管的血管舒张有影响。在包绕浆液细胞和导管细胞的众多肌上皮细胞以及腺体内血管的平滑肌细胞中可见肌动蛋白免疫反应性。通过使用抗P物质血清和抗肌动蛋白血清的双重免疫标记技术，发现P物质免疫反应性曲张神经纤维与肌上皮细胞紧密接触。
