Piérard G E, Goffin V, Hermanns-Lê T, Arrese J E, Piérard-Franchimont C
Department of Dermatopathology, University of Liège, Belgium.
J Am Acad Dermatol. 1995 Sep;33(3):462-9. doi: 10.1016/0190-9622(95)91392-0.
Surfactants elicit alterations in the stratum corneum. Predictive tests that avoid animal experimentation are needed.
This study compares three methods of rating and predicting shampoo-induced irritation.
Corneosurfametry entails collection of stratum corneum followed by brief contact with diluted surfactants and measurement of variations in staining of samples.
Corneosurfametry appears to correlate well with in vivo testing in volunteers with sensitive skin. However, corneosurfametry presents less interindividual variability than in vivo testing and allows better discrimination among mild products. Morphologic information about surfactant-induced loosening of corneocytes may be increased by testing surfactants on human skin equivalent. Results are similar to those provided by specimens used for corneosurfametry.
The corneosurfametric prediction of surfactant irritancy correlates with in vivo testing and with in vitro evaluation on human skin equivalent.
表面活性剂会引起角质层的变化。需要避免动物实验的预测性测试。
本研究比较了三种评估和预测洗发水引起的刺激性的方法。
角质层表面活性测定法需要收集角质层，然后使其与稀释的表面活性剂短暂接触，并测量样品染色的变化。
角质层表面活性测定法似乎与敏感性皮肤志愿者的体内测试有很好的相关性。然而，角质层表面活性测定法的个体间变异性比体内测试小，并且能更好地区分温和产品。通过在人皮替代品上测试表面活性剂，可能会增加有关表面活性剂引起的角质形成细胞松解的形态学信息。结果与用于角质层表面活性测定法的标本所提供的结果相似。
表面活性剂刺激性的角质层表面活性测定预测与体内测试以及人皮替代品上的体外评估相关。
