Piérard G E, Goffin V, Piérard-Franchimont C
Department of Dermatopathology, University of Liège, Belgium.
Dermatology. 1994;189(2):152-6. doi: 10.1159/000246820.
Corneosurfametry is introduced as a noninvasive quantitative test rating the interaction between surfactants and human stratum corneum. It may be used as a predictive irritancy test.
Surfactants present in personal-care products elicit multiple effects on the stratum corneum. With upcoming regulations avoiding animal experiments and ethical considerations for human testing, there is a need for new in vitro methods evaluating irritancy.
Corneosurfametry entails collection of cyanoacrylate skin surface strippings and short contact time with surfactants followed by staining samples with toluidine blue and basic fuchsin dyes. Measurements are made by reading the color of samples using reflectance colorimetry.
The intensity of color increases with irritancy potential of the surfactant. Results are reproducible, and great differences are noted among a series of diluted shampoos, shower gels and facial cleansing gels.
Corneosurfametry is proposed as a rapid in vitro method allowing a predictive grading of surfactants related to irritancy.
角膜表面活性测定法是一种无创定量检测方法，用于评估表面活性剂与人体角质层之间的相互作用。它可用作预测刺激性的测试。
个人护理产品中的表面活性剂对角质层有多种影响。随着即将出台的法规避免动物实验以及出于人体测试的伦理考虑，需要新的体外方法来评估刺激性。
角膜表面活性测定法包括收集氰基丙烯酸酯皮肤表面剥离物，并使其与表面活性剂短时间接触，然后用甲苯胺蓝和碱性品红染料对样本进行染色。通过反射比色法读取样本颜色进行测量。
颜色强度随表面活性剂的刺激潜力增加而增加。结果具有可重复性，并且在一系列稀释的洗发水、沐浴露和洁面凝胶之间存在显著差异。
角膜表面活性测定法被提议作为一种快速的体外方法，可对与刺激性相关的表面活性剂进行预测分级。
