Stepanova Amaliya, de Hoog G Sybren, Vasilyeva Nataliya, Raznatovskiy Konstantin, Chilina Galina
North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia.
Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
Curr Med Mycol. 2020;6(1):42-46. doi: 10.18502/cmm.6.1.2508.
is a widely distributed anthropophilic dermatophyte causing different diseases of skin. In the literature limited data are available about the morphogenesis of vegetative mycelium of and related anthropophilic dermatophytes. The aim of present study was to describe ultrastructural patterns of development, cellular organellography and septal pore apparatus structure of growing vegetative mycelium of .
strain RCPFF 214/898 was grown on solid Czapek's Agar (CzA) at 28ºС. For investigation of colonies morphology we used methods of light-, scanning and transmission electron microscopy (SEM and TEM).
Differences in morphogenesis of aerial and substrate hyphae were revealed. Mitochondrial reticulum and fibrosinous bodies were shown in for the first time. The septal pore apparatus in hyphal cells of was comprised Woronin bodies and septal pore plugs. Woronin bodies (0.18 µm), located with 1‒4 near the pore, were spherical, membrane-bound, and had a homogeneous, electron-dense content. The cells of aerial and submerged hyphal cells of contain two nuclei.
Mature cells of substrate hyphae appeared more active than comparable cells in the aerial mycelium. During the maturation process, the differences in number and morphology of mitochondria, number of vacuoles, and in the synthesis of different types of storage substances were revealed. Presence of "mitochondrial reticulum" and variable types of storage substances in submerged hyphal cells suggested higher levels of metabolic activity compared to aerial mycelium.
是一种广泛分布的嗜人皮肤癣菌，可引起多种皮肤疾病。关于该菌及相关嗜人皮肤癣菌的营养菌丝形态发生，文献中的数据有限。本研究旨在描述生长中的营养菌丝的超微结构发育模式、细胞器图谱及隔膜孔装置结构。
菌株RCPFF 214/898在28℃的固体察氏琼脂（CzA）上培养。为研究菌落形态，我们采用了光镜、扫描电子显微镜和透射电子显微镜（SEM和TEM）方法。
揭示了气生菌丝和基内菌丝形态发生的差异。首次在该菌中发现线粒体网状结构和纤维状小体。该菌菌丝细胞中的隔膜孔装置由沃罗宁体和隔膜孔塞组成。沃罗宁体（0.18 µm），在孔附近1 - 4个位置，呈球形，有膜包裹，内容物均匀、电子密度高。气生菌丝和 submerged hyphal cells（此处原文有误，推测可能是substrate hyphal cells，即基内菌丝）的细胞含有两个细胞核。
基内菌丝的成熟细胞似乎比气生菌丝中的同类细胞更活跃。在成熟过程中，揭示了线粒体数量和形态、液泡数量以及不同类型储存物质合成方面的差异。基内菌丝细胞中存在“线粒体网状结构”和多种类型的储存物质，表明其代谢活性水平高于气生菌丝。
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