Keller R, Keist R, Joller P
Department of Pathology, University of Zurich, Switzerland.
Scand J Immunol. 1995 Sep;42(3):337-44. doi: 10.1111/j.1365-3083.1995.tb03665.x.
The ability of inactivated viruses, bacteria, protozoa and fungi to modulate the expression of CD14, CD49d, CD49f, CD11a (LFA-1), and CD54 (ICAM-1) molecules in unprimed bone marrow-derived mononuclear phagocytes (BMM phi) was investigated by means of flow cytometry. Incubation with bacterial agents resulted in the large majority of experimental situations in enhanced expression of these macrophage surface molecules. In contrast, viruses and fungi down-regulated the expression of several adhesion receptors, especially integrins. Amplification of MHC class II expression triggered in macrophages by interferon gamma was clearly inhibited by viruses, bacteria, protozoa and fungi. The findings explain earlier results showing that, under the same experimental conditions, bacterial agents are, for the most part, potent stimulators of secretory and cell-mediated macrophage activities while viruses, protozoa and fungi are poor in this respect.
通过流式细胞术研究了灭活病毒、细菌、原生动物和真菌调节未致敏骨髓来源的单核吞噬细胞（BMM phi）中CD14、CD49d、CD49f、CD11a（LFA-1）和CD54（ICAM-1）分子表达的能力。在大多数实验情况下，与细菌制剂孵育会导致这些巨噬细胞表面分子的表达增强。相比之下，病毒和真菌下调了几种粘附受体的表达，尤其是整合素。干扰素γ在巨噬细胞中引发的MHC II类表达的放大明显受到病毒、细菌、原生动物和真菌的抑制。这些发现解释了早期的结果，即在相同的实验条件下，细菌制剂在很大程度上是分泌性和细胞介导的巨噬细胞活性的有效刺激物，而病毒、原生动物和真菌在这方面则较弱。
