Keller R, Gehri R, Keist R
Department of Pathology, University Hospital, Zurich, Switzerland.
Cell Immunol. 1994 Dec;159(2):323-30. doi: 10.1006/cimm.1994.1318.
The secretory (tumor necrosis factor, TNF-alpha; nitrite) and cellular response (mitochondrial respiration, TNF-alpha-independent tumoricidal activity) of a pure, lymphocyte-free population of resting, unprimed rat bone marrow-derived mononuclear phagocytes (BMM phi) to direct interaction with viruses, protozoa, and fungi was assessed and compared with that triggered by bacterial agents and interferon-gamma (IFN-gamma). Viruses (herpes simplex, vaccinia, poliomyelitis, vesicular stomatitis, lymphocytic choriomeningitis, Sendai), protozoa (Trypanosoma brucei, Giardia lamblia), and fungi (Penicillium, Trichosporon, Fusarium, Rhizopus, Aspergillus, Geotrichum species) affected primarily the secretion of TNF-alpha and mitochondrial respiration of BMM phi; their effects on the secretion of nitrite and on tumoricidal activity were at best marginal. Collectively, the macrophage response to viruses, protozoa, and fungi was less varied and less marked than that to bacterial agents (intact organisms, peptidoglycan, lipoteichoic acid, lipopolysaccharide) and IFN-gamma.
评估了静息、未致敏的大鼠骨髓来源单核吞噬细胞（BMM phi）的纯淋巴细胞无群体与病毒、原生动物和真菌直接相互作用时的分泌反应（肿瘤坏死因子，TNF-α；亚硝酸盐）和细胞反应（线粒体呼吸，TNF-α非依赖性杀肿瘤活性），并与细菌制剂和干扰素-γ（IFN-γ）引发的反应进行了比较。病毒（单纯疱疹病毒、痘苗病毒、脊髓灰质炎病毒、水泡性口炎病毒、淋巴细胞性脉络丛脑膜炎病毒、仙台病毒）、原生动物（布氏锥虫、蓝氏贾第鞭毛虫）和真菌（青霉属、毛孢子菌属、镰刀菌属、根霉属、曲霉属、地丝菌属菌种）主要影响BMM phi的TNF-α分泌和线粒体呼吸；它们对亚硝酸盐分泌和杀肿瘤活性的影响至多微乎其微。总体而言，巨噬细胞对病毒、原生动物和真菌的反应比其对细菌制剂（完整生物体、肽聚糖、脂磷壁酸、脂多糖）和IFN-γ的反应变化更小且不那么明显。
