Baert J H, Viaene A I, Veys R J
Laboratory of Histology, University of Leuven, Kortrijk, Belgium.
Acta Anat (Basel). 1995;152(2):85-92. doi: 10.1159/000147687.
In this hybridohistochemical study, we investigated the expression of cytokeratin (CK) mRNA in the keratinizing squamous epithelium of hamster cheek pouch and esophagus, using eight different digoxigenin-labelled RNA probes complementary to human CK mRNAs. CK 4, CK 6, CK 8, CK 14, and CK 15 RNA probe obviously hybridized with hamster counterpart mRNA(s) in the cheek pouch as well as in the esophageal epithelium. However, using the CK 10-specific RNA probe, only the cheek pouch epithelium exhibited a positive reaction. We were not able to detect any positive signal for the CK 18 or for the CK 19 RNA probe. We observed three different CK mRNA distribution patterns in the cheek pouch epithelium, and four in the esophageal epithelium. The differences in expression and distribution pattern of CK mRNAs between the two types of epithelia suggest that the hamster CK polypeptide family comprises at least six different species. We also conclude that human cRNA probes for CK mRNAs may provide a way to detect changes in CK expression and distribution during induced non-neoplastic and neoplastic changes in the hamster cheek pouch model. This may also help to elucidate the molecular pathogenesis of squamous-cell carcinoma.
在这项杂交组织化学研究中，我们使用了八种与人细胞角蛋白（CK）mRNA互补的不同地高辛标记RNA探针，研究了仓鼠颊囊和食管角质化鳞状上皮中CK mRNA的表达情况。CK 4、CK 6、CK 8、CK 14和CK 15 RNA探针在颊囊以及食管上皮中明显与仓鼠相应的mRNA杂交。然而，使用CK 10特异性RNA探针时，仅颊囊上皮呈现阳性反应。我们未能检测到CK 18或CK 19 RNA探针的任何阳性信号。我们在颊囊上皮中观察到三种不同的CK mRNA分布模式，在食管上皮中观察到四种。两种上皮中CK mRNA表达和分布模式的差异表明，仓鼠CK多肽家族至少包含六种不同的类型。我们还得出结论，用于CK mRNA的人cRNA探针可能为检测仓鼠颊囊模型中诱导的非肿瘤性和肿瘤性变化过程中CK表达和分布的变化提供一种方法。这也可能有助于阐明鳞状细胞癌的分子发病机制。
