Westwood N B, Copson E R, Page L A, Mire-Sluis A R, Brown K A, Pearson T C
Division of Haematology, United Medical School, Guys' Hospital, London.
J Clin Pathol. 1995 Jun;48(6):525-30. doi: 10.1136/jcp.48.6.525.
To investigate whether monocytes and neutrophils from patients with primary proliferative polycythaemia (PPP) exhibit increased expression of markers of cell activation and, if so, whether they are associated with the phagocytic activity of these cells and concentrations of circulating cytokines.
Expression of CD11b, CD14, CD18, and CD64 on monocytes and neutrophils was assessed by flow cytometry. Phagocytosis was analysed using immunoglobulin opsonised Escherichia coli. Serum concentrations of granulocyte colony stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF) and macrophage CSF (M-CSF) were determined by bioassays, and interferon-gamma (IFN-gamma) by enzyme linked immunosorbent assay (ELISA).
Patients with PPP (n = 18), when compared with normal subjects (n = 10), had increased percentages of CD64+ monocytes (52% v 36%) and neutrophils (42% v 11%) and of CD14+ neutrophils (36% v 18%). Monocytes from patients with PPP exhibited increased expression of CD64 (47 v 26) and of CD11b (65 v 36). These abnormalities were not found in patients with secondary (n = 8) or apparent (n = 13) polycythaemia. The percentage of neutrophils undergoing phagocytosis was higher in patients with PPP (mean 64%; n = 6) than in normal subjects (mean 42%; n = 5). G-CSF, GM-CSF and IFN-gamma concentrations in patients' serum samples were comparable with normal; M-CSF was not detected in any of the samples. There was no correlation between cytokine concentrations and the expression of CD11b, CD14, CD18, and CD64 on patients' phagocytes.
Increased expression of CD11b and CD64 by monocytes, increased percentages of CD14+ and CD64+ neutrophils and the high phagocytic activity of neutrophils suggests that these cells are activated in vivo in patients with PPP. The phenotypic changes of PPP phagocytes were not associated with increased concentrations of circulating cytokines and probably reflect intrinsic abnormalities within the neoplastic PPP clone.
研究原发性增殖性红细胞增多症（PPP）患者的单核细胞和中性粒细胞是否表现出细胞活化标志物表达增加，若如此，这些变化是否与这些细胞的吞噬活性及循环细胞因子浓度相关。
采用流式细胞术评估单核细胞和中性粒细胞上CD11b、CD14、CD18和CD64的表达。使用免疫球蛋白调理的大肠杆菌分析吞噬作用。通过生物测定法测定血清中粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和巨噬细胞集落刺激因子（M-CSF）的浓度，通过酶联免疫吸附测定法（ELISA）测定干扰素-γ（IFN-γ）的浓度。
与正常受试者（n = 10）相比，PPP患者（n = 18）中CD64+单核细胞（52%对36%）和中性粒细胞（42%对11%）以及CD14+中性粒细胞（36%对18%）的百分比增加。PPP患者的单核细胞表现出CD64（47对26）和CD11b（65对36）表达增加。继发性（n = 8）或相对性（n = 13）红细胞增多症患者未发现这些异常。PPP患者（平均64%；n = 6）中发生吞噬作用的中性粒细胞百分比高于正常受试者（平均42%；n = 5）。患者血清样本中的G-CSF、GM-CSF和IFN-γ浓度与正常水平相当；所有样本中均未检测到M-CSF。细胞因子浓度与患者吞噬细胞上CD11b、CD14、CD18和CD64的表达之间无相关性。
单核细胞CD11b和CD64表达增加、CD14+和CD64+中性粒细胞百分比增加以及中性粒细胞的高吞噬活性表明，PPP患者体内这些细胞被激活。PPP吞噬细胞的表型变化与循环细胞因子浓度增加无关，可能反映了肿瘤性PPP克隆内的内在异常。
