Mutagenesis. 1995 May;10(3):153-9. doi: 10.1093/mutage/10.3.153.
Although the target cells for the bone marrow (BM) and peripheral blood (PB) micronucleus tests are the same, erythroblasts, the PB method offers important advantages over the BM method. We propose a protocol for the short-term peripheral blood micronucleus test. This assay is intended primarily for the identification of a wide variety of chemical clastogens and spindle poisons, and secondarily for risk assessment. The recommended experiment size, seemingly small, has adequate detection power. Experimental results obtained from Collaborative Study Group for the Micronucleus Test (CSGMT) studies and data collected from a survey of the literature provided the basis of the proposed protocol. Our protocol, designed for mice, includes the following features. (i) The maximum tolerated dose (MTD) is determined experimentally with a small number of animals treated i.p. or per os (or by other routes, if called for) in a dose-finding test, which can be conducted simultaneously with tests for finding both number of treatments and optimal sampling time. (ii) At least three groups of five mice (at least four effective animals per group), males or females, are given i.p. or per os doses of, for example, the MTD, 1/2 MTD and 1/4 MTD once, twice or more, 24 h apart. (iii) Peripheral blood samples are taken before treatment (the 0 time control) and twice at 48 and 72 h for a single treatment, once between 24 and 36 h after the second treatment for double treatments, or once 24 h after the final treatment for multiple dosing. Or, if an optimal sample time is established in a preliminary test, samples are taken at that time, (iv) Samples are stained with acridine orange, and 2000 immature erythrocytes per animal are examined. (v) The combined data of 0 time samples are the negative control.(ABSTRACT TRUNCATED AT 250 WORDS)
虽然骨髓（BM）和外周血（PB）微核试验的靶细胞相同，均为成红细胞，但PB法相对于BM法具有重要优势。我们提出了一种短期外周血微核试验方案。该检测主要用于识别多种化学断裂剂和纺锤体毒物，其次用于风险评估。推荐的实验规模看似较小，但具有足够的检测能力。微核试验协作研究组（CSGMT）研究获得的实验结果以及从文献调查收集的数据为所提出的方案提供了依据。我们为小鼠设计的方案包括以下特点。（i）通过在剂量探索试验中对少量经腹腔注射或经口（或根据需要通过其他途径）处理的动物进行实验确定最大耐受剂量（MTD），该试验可与确定处理次数和最佳采样时间的试验同时进行。（ii）至少三组，每组五只小鼠（每组至少四只有效动物），雄性或雌性，经腹腔注射或经口给予例如MTD、1/2 MTD和1/4 MTD的剂量，一次、两次或更多次，间隔24小时。（iii）在处理前（0时间对照）采集外周血样本，单次处理时在48小时和72小时采集两次，两次处理时在第二次处理后24至36小时采集一次，多次给药时在最后一次处理后24小时采集一次。或者，如果在初步试验中确定了最佳采样时间，则在该时间采集样本。（iv）样本用吖啶橙染色，每只动物检查2000个未成熟红细胞。（v）0时间样本的合并数据为阴性对照。（摘要截断于250字）
