生物素化透明质酸作为检测细胞和组织中结合蛋白的探针。
Biotinylated hyaluronan as a probe for detection of binding proteins in cells and tissues.
作者信息
Yu Q, Toole B P
机构信息
Tufts University School of Medicine, Boston, MA, USA.
出版信息
Biotechniques. 1995 Jul;19(1):122-4, 126-9.
PMID:7545407
Abstract
A convenient and reliable method for preparing and using biotinylated hyaluronan for detection of hyaluronan-binding proteins is described. The biotinylated hyaluronan can be used to detect binding proteins in transblots after electrophoresis or as a histological probe for localization of binding proteins in cultured cells and tissue sections.
摘要
本文描述了一种制备和使用生物素化透明质酸以检测透明质酸结合蛋白的便捷可靠方法。生物素化透明质酸可用于电泳后转印杂交中检测结合蛋白，或作为组织学探针用于定位培养细胞和组织切片中的结合蛋白。
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