Hoare K, Savani R C, Wang C, Yang B, Turley E A
Department of Pediatrics and Child Health, Manitoba Institute of Cell Biology, Winnipeg, Canada.
Connect Tissue Res. 1993;30(2):117-26. doi: 10.3109/03008209309041327.
A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.
描述了一种在蛋白质印迹分析中使用生物素化透明质酸（BHA）检测透明质酸（HA）结合蛋白的方法。利用该分析方法对一种名为RHAMM（HA介导运动的受体）的新型HA受体的一些结合特性进行了表征。该方法还用于检测组织匀浆中的其他HA结合蛋白。此方法具有半定量、快速、可重复、灵敏的特点，因此在鉴定不同细胞和组织中HA结合蛋白水平方面具有潜在用途。
