Adonogianaki E, Mooney J, Wennström J L, Lekholm U, Kinane D F
Department of Adult Dental Care, Glasgow Dental Hospital and School, University of Glasgow, Scotland, United Kingdom.
Clin Oral Implants Res. 1995 Mar;6(1):14-23. doi: 10.1034/j.1600-0501.1995.060102.x.
This investigation had 2 aims: 1) to determine the levels of acute-phase proteins and immunoglobulin G (IgG) against Porphyromonas gingivalis in peri-implant crevicular fluid (PICF) and their association with the clinical condition of the peri-implant mucosa; and 2) to compare the inflammatory and immunological responses at implants and teeth as reflected by the gingival crevicular fluid (GCF) and PICF levels of acute-phase proteins and immunoglobulins. Thirty-one partially edentulous subjects were recruited for this study. PICF was sampled from 1 healthy and 1 inflamed site from each patient; GCF was sampled from an additional 21 healthy and 27 inflamed tooth sites of the same patients. GCF and PICF were collected with paper strips (for 30 s) and analysed using enzyme-linked immunosorbent assays for alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin, lactoferrin and IgG against P. gingivalis. This investigation demonstrated that the absolute amounts of the acute-phase proteins and IgG against P. gingivalis are higher in GCF and PICF from inflamed than healthy sites. No significant differences were observed between PICF and GCF components at either healthy or inflamed sites, suggesting that inflammatory and immune events are similar in the peri-implant mucosa and gingiva in humans and that PICF and GCF production is governed by similar mechanisms.
1）确定种植体周围龈沟液（PICF）中急性期蛋白和抗牙龈卟啉单胞菌免疫球蛋白G（IgG）的水平及其与种植体周围黏膜临床状况的关联；2）比较种植体和牙齿处由龈沟液（GCF）和PICF中急性期蛋白及免疫球蛋白水平所反映的炎症和免疫反应。31名部分牙列缺损受试者被纳入本研究。从每位患者的1个健康部位和1个炎症部位采集PICF；从同一患者额外的21个健康牙齿部位和27个炎症牙齿部位采集GCF。用纸条收集GCF和PICF（持续30秒），并使用酶联免疫吸附测定法分析α2-巨球蛋白、α1-抗胰蛋白酶、转铁蛋白、乳铁蛋白以及抗牙龈卟啉单胞菌的IgG。本研究表明，来自炎症部位的GCF和PICF中，急性期蛋白和抗牙龈卟啉单胞菌IgG的绝对量高于健康部位。在健康或炎症部位，PICF和GCF成分之间均未观察到显著差异，这表明人类种植体周围黏膜和牙龈中的炎症和免疫事件相似，且PICF和GCF的产生受相似机制调控。
