Mooney J, Kinane D F
Periodontal Unit, Glasgow University Dental School, United Kingdom.
Oral Microbiol Immunol. 1997 Apr;12(2):112-6. doi: 10.1111/j.1399-302x.1997.tb00626.x.
Titers of immunoglobulin G (IgG) directed against Porphyromonas gingivalis in gingival crevicular fluid of 40 periodontitis patients were measured at three sites in each patient (healthy, gingivitis and periodontitis) by enzyme-linked immunosorbent assay. When paired analyses were performed using Wilcoxon signed-rank tests, periodontitis sites were found to have lower median titers than gingivitis sites. Both systemic and locally-produced antibodies contribute to the overall gingival crevicular fluid antibody profile. Albumin, in contrast, is derived only from serum, and thus this protein serves as an indicator of serum contribution to gingival crevicular fluid. Correction was therefore made for systemic input to the gingival crevicular fluid IgG profile by expressing the results per unit of albumin. When this was done, periodontitis sites were also found to have significantly lower antibody levels than gingivitis sites. These findings suggest that a failure of local antibody production or reduction in quantities, by, for example, degradation by bacterial proteases, may contribute to the change from a gingivitis to a periodontitis lesion.
通过酶联免疫吸附测定法，在40名牙周炎患者的每个患者的三个部位（健康、牙龈炎和牙周炎）测量龈沟液中针对牙龈卟啉单胞菌的免疫球蛋白G（IgG）滴度。当使用Wilcoxon符号秩检验进行配对分析时，发现牙周炎部位的中位数滴度低于牙龈炎部位。全身和局部产生的抗体都对龈沟液中的总体抗体谱有贡献。相比之下，白蛋白仅来源于血清，因此这种蛋白质可作为血清对龈沟液贡献的指标。因此，通过以每单位白蛋白表示结果，对龈沟液IgG谱的全身输入进行了校正。当这样做时，还发现牙周炎部位的抗体水平明显低于牙龈炎部位。这些发现表明，局部抗体产生失败或数量减少，例如被细菌蛋白酶降解，可能导致从牙龈炎病变转变为牙周炎病变。
