Okuyama R, Koguma M, Yanai N, Obinata M
Department of Cell Biology, Tohoku University, Sendai, Japan.
Blood. 1995 Oct 1;86(7):2590-7.
Regulation of development of hematopoietic stem cells was examined by culturing Lin- c-Kit+ Sca1+ stem cells sorted from bone marrow (BM) cells by fluorescence-activated cell sorting on a layer of TBR59, a BM stromal cell line established from simian virus 40 T-antigen gene transgenic mice. The sorted stem cells did not show self-renewal, but two waves (at 7 and 13 days) of a cobblestone formation were induced by the stromal cell layer. The cobblestones were formed by finite cell division (eight divisions on average) of sorted Lin- c-Kit+ Sca1+ stem cells, and divided cells were still immature. The c-Kithigh stem cell population was induced to form the first wave of cobblestone formation committed to myeloid lineage, whereas c-Kitlow population was induced to form the second wave of this formation committed to lymphoid lineage. Both cobblestone formations require c-Kit function, but very late activation antigen-4-vascular cell adhesion molecule-1 interaction plays different parts in the two lineages.
通过对利用荧光激活细胞分选从骨髓（BM）细胞中筛选出的Lin-c-Kit+ Sca1+干细胞在TBR59层上进行培养，来研究造血干细胞的发育调控。TBR59是一种从猿猴病毒40 T抗原基因转基因小鼠建立的BM基质细胞系。分选的干细胞未显示自我更新能力，但基质细胞层诱导产生了两波（分别在第7天和第13天）鹅卵石样结构的形成。鹅卵石样结构由分选的Lin-c-Kit+ Sca1+干细胞通过有限的细胞分裂（平均八次分裂）形成，且分裂后的细胞仍未成熟。c-Kithigh干细胞群体被诱导形成第一波致力于髓系谱系的鹅卵石样结构形成，而c-Kitlow群体被诱导形成第二波致力于淋巴系谱系的此结构形成。两波鹅卵石样结构的形成均需要c-Kit功能，但极晚期活化抗原-4-血管细胞黏附分子-1相互作用在两个谱系中发挥不同作用。
