Li C L, Johnson G R
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Blood. 1995 Mar 15;85(6):1472-9.
Murine bone marrow cells were fractionated by fluorescence-activated cell sorting into Rh123lo Lin- c-kit+ Ly6A+, Rh123hi Lin-c-kit+ Ly6A+, and Lin- c-kit+ Ly6A- populations within which most, if not all, of the hematopoietic activities of the marrow resided. The Rh123lo Lin- c-kit+Ly6A+ cells, which consist exclusively of small- or medium-sized lymphocyte-like cells, are highly enriched for long-term hematopoietic in vivo repopulating cells. The enrichment factor for these cells from the marrow was estimated as 2,000-fold. The Rh123hi Lin- c-kit+ Ly6A+ cells, although also highly enriched for day-12 spleen colony-forming units, were relatively depleted of long-term in vivo repopulation capacity. Most, if not all Lin- c-kit+ Ly6A- cells were Rb123hi. In contrast to both Rh123lo and Rh123hi Lin- c-kit+ Ly6A+ stem cell populations, the Lin- c-kit+ Ly6A- cells can be stimulated to proliferate in vitro in the presence of single cytokines, which is a characteristic of committed progenitor cells. No marked synergistic interactions between individual cytokines were observed with this cell population. Both Rh123hi Lin- c-kit+ Ly6A+ mature stem cell and Lin- c-kit+ Ly6A- progenitor cell populations displayed in vivo repopulation kinetics resembling those of the putative short-term hematopoietic repopulating cells.
通过荧光激活细胞分选法将小鼠骨髓细胞分离为Rh123lo Lin-c-kit+ Ly6A+、Rh123hi Lin-c-kit+ Ly6A+和Lin-c-kit+ Ly6A-群体，骨髓的大部分（如果不是全部）造血活性存在于这些群体中。Rh123lo Lin-c-kit+Ly6A+细胞仅由小或中等大小的淋巴细胞样细胞组成，高度富集体内长期造血再填充细胞。这些细胞从骨髓中的富集因子估计为2000倍。Rh123hi Lin-c-kit+ Ly6A+细胞虽然也高度富集第12天脾集落形成单位，但体内长期再填充能力相对不足。大多数（如果不是全部）Lin-c-kit+ Ly6A-细胞为Rb123hi。与Rh123lo和Rh123hi Lin-c-kit+ Ly6A+干细胞群体不同，Lin-c-kit+ Ly6A-细胞在单一细胞因子存在下可在体外被刺激增殖，这是定向祖细胞的一个特征。该细胞群体未观察到单个细胞因子之间有明显的协同相互作用。Rh123hi Lin-c-kit+ Ly6A+成熟干细胞和Lin-c-kit+ Ly6A-祖细胞群体在体内的再填充动力学类似于假定的短期造血再填充细胞。
