Isobe K, Imoto M, Fukuda Y, Koyama Y, Nakano I, Hayakawa T, Takamatsu J
Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Liver. 1995 Jun;15(3):131-4. doi: 10.1111/j.1600-0676.1995.tb00659.x.
Liver function and antibodies to hepatitis C virus and to human immunodeficiency virus-1 were examined in 195 Japanese patients with hemophilia. One hundred and seventy-three were positive for antibody to HCV and 61 for antibody to human immunodeficiency virus-1. In 63 patients, we examined HCV genotypes according to the double polymerase chain reaction method. Forty cases (63%) were infected with hepatitis C virus with a single genotype, including type 1a in five, type 1b in 21, type 2a in seven and type 2b in seven; 16 (25%) were infected with double genotypes, including types 1a + 1b in 14, types 1b + 2a in one and types 1b+2b in one; and four (6%) were infected with triple genotypes, including types 1a + 1b + 2b in two. Genotype could not be determined in three patients by this method. In the 191 nonhemophiliac patients with chronic hepatitis C, HCV genotyping was as follows: type 1a in 0, type 1b in 121, type 2a in 40 and type 2b in 10 of 171 cases (89.5%) with single infection and types 1b + 2a in five and types 2a + 2b in one of six (5.5%) with double infection. In the remaining 14 patients, genotype could not be determined. Frequent transfusion of domestic and/or imported coagulation factor concentrates probably caused the high incidence of HCV infection with rare or mixed genotypes in Japanese hemophiliacs.
对195名日本血友病患者进行了肝功能以及丙型肝炎病毒抗体和人类免疫缺陷病毒1型抗体检测。173人丙型肝炎病毒抗体呈阳性，61人人类免疫缺陷病毒1型抗体呈阳性。我们采用双重聚合酶链反应法对63例患者检测了丙型肝炎病毒基因型。40例（63%）感染单一基因型丙型肝炎病毒，其中1a型5例、1b型21例、2a型7例、2b型7例；16例（25%）感染双重基因型，其中1a + 1b型14例、1b + 2a型1例、1b + 2b型1例；4例（6%）感染三重基因型，其中1a + 1b + 2b型2例。3例患者用此方法无法确定基因型。在191例非血友病慢性丙型肝炎患者中，丙型肝炎病毒基因分型情况如下：171例单一感染患者中，1a型0例、1b型121例、2a型40例、2b型10例（89.5%）；6例双重感染患者中，1b + 2a型5例、2a + 2b型1例（5.5%）。其余14例患者无法确定基因型。频繁输注国产和/或进口凝血因子浓缩剂可能导致日本血友病患者中丙型肝炎病毒感染率高，且基因型罕见或呈混合状态。
