Tawaraya H, Ohkoshi S, Kuwana K, Watanabe M, Kamimura T, Asakura H
Third Department of Internal Medicine, School of Medicine, Niigata University, Japan.
J Med Virol. 1995 Apr;45(4):367-72. doi: 10.1002/jmv.1890450403.
Mass screening for hepatitis C virus antibody was carried out in 875 inhabitants (313 men and 562 women) of a town in Japan with a high rate of hepatitis B virus infection. The overall rate of positivity for anti-HCV was 8.8% (6.4% in men and 10.1% in women). The rate of positivity for hepatitis B virus surface antigen was 11.2%. Five subjects (0.6%) were positive for both markers. HCV-RNA was detected in 65 (88.4%) of 77 individuals who were positive for anti-HCV and in 1 (1.5%) of 60 individuals negative for anti-HCV. The genotype of the HCV genome was determined by PCR analysis using type-specific primers in 60 individuals. HCV type 1b was detected in 51 subjects (85%), type 2a in 3 subjects (5%), and type 2b in 6 subjects (10%). None of the individuals was infected with more than one genotype. The nucleotide sequences of the partial nonstructural 5 region of HCV type 1b genotype obtained from 6 individuals showed at least 92.0% homology in the nucleotide sequence, and 94.8% homology in the amino acid sequence. Homology among these clones was greater than their homology with previously described type 1b sequences. The findings suggest that there was a specific local origin of HCV infection, although it was not possible to identify any single source of HCV infection. The results also indicate that presence of asymptomatic HCV carriers.
在日本一个乙肝病毒感染率较高的城镇，对875名居民（313名男性和562名女性）进行了丙型肝炎病毒抗体的大规模筛查。抗-HCV总体阳性率为8.8%（男性为6.4%，女性为10.1%）。乙肝病毒表面抗原阳性率为11.2%。5名受试者（0.6%）两种标志物均呈阳性。在77名抗-HCV阳性个体中的65名（88.4%）以及60名抗-HCV阴性个体中的1名（1.5%）检测到HCV-RNA。使用型特异性引物通过PCR分析确定了60名个体中HCV基因组的基因型。在51名受试者（85%）中检测到HCV 1b型，3名受试者（5%）中检测到2a型，6名受试者（10%）中检测到2b型。没有个体感染超过一种基因型。从6名个体获得 的HCV 1b基因型部分非结构5区的核苷酸序列在核苷酸序列中显示至少92.0%的同源性，在氨基酸序列中显示94.8%的同源性。这些克隆之间的同源性大于它们与先前描述的1b型序列的同源性。这些发现表明，尽管无法确定任何单一的HCV感染源，但存在特定的HCV感染本地起源。结果还表明存在无症状HCV携带者。
