Dupuy P
Ann Nutr Aliment. 1978;32(5):1123-32.
In many countries addition of sugar to the grape must for increasing the alcohol concentration is autorized by regulation. This addition must be supervised by a priori and a posteriori controls. The saccharose from sugar beet contrains 100 mg/kg of betain, which can be determined in wine after purification by ion exchange and gas chromatography of a decomposition product of its butylester. Methyl betaine has been used as internal standard to improve the method. The natural wine contains low quantity of betaine. For this reason it is impossible to detect an addition of sugar lower than that corresponding to 2 degrees alcohol. The other methods (13C content of ethanol, polyosides contained as impurity in sugar) seem to present the same limitation.
在许多国家,通过法规允许在葡萄汁中添加糖分以提高酒精浓度。这种添加必须通过事前和事后控制进行监管。甜菜糖含有100毫克/千克的甜菜碱,可在通过离子交换和其丁酯分解产物的气相色谱法纯化后在葡萄酒中测定。甲基甜菜碱已被用作内标以改进该方法。天然葡萄酒中甜菜碱含量较低。因此,无法检测到低于相当于2度酒精的糖分添加量。其他方法(乙醇的13C含量、糖中作为杂质所含的多聚糖)似乎也存在同样的局限性。