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一种感染十字花科植物的烟草花叶病毒属病毒的完整核苷酸序列及基因组结构

Complete nucleotide sequence and genome organization of a tobamovirus infecting cruciferae plants.

作者信息

Ivanov P A, Novikov V K, Agranovsky A A, Efimov V A, Casper R, Atabekov J G

机构信息

A.N. Belozersky Institute of Physico-Chemical Biology, Moscow, Russian Federation.

出版信息

FEBS Lett. 1994 Aug 15;350(1):5-8. doi: 10.1016/0014-5793(94)00721-7.

DOI:10.1016/0014-5793(94)00721-7
PMID:7545946
Abstract

Genomic RNA sequence of a tobamovirus infecting cruciferae plants (cr-TMV) was determined. The RNA is composed of 6312 nucleotides and contains four ORFs encoding the proteins of 122K (ORF1), 178K (ORF2), 29K (ORF3) and 18K (capsid protein, ORF4). ORF4 overlaps ORF3 by 74 nucleotides and the overlapping region can be folded into a stable hairpin structure. The 3'-terminal region of the cr-TMV RNA preceding the tRNA-like structure was shown to form six potentially stable pseudoknots.

摘要

确定了一种感染十字花科植物的烟草花叶病毒（cr-TMV）的基因组RNA序列。该RNA由6312个核苷酸组成，包含四个开放阅读框，分别编码122K（开放阅读框1）、178K（开放阅读框2）、29K（开放阅读框3）和18K（衣壳蛋白，开放阅读框4）的蛋白质。开放阅读框4与开放阅读框3重叠74个核苷酸，重叠区域可折叠成稳定的发夹结构。cr-TMV RNA的3'末端区域在类似tRNA结构之前显示可形成六个潜在稳定的假结。

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