人淋巴细胞与DNA孵育后中性和酸性DNA酶的分泌；对吸入麻醉的可能影响。
Secretion of neutral and acid DNases in cultivated human lymphocytes after incubation with DNA; possible consequences for inhalation anesthesia.
作者信息
Reitz M, Löber G, Kleemann P, Dick W
机构信息
Klinik für Anästhesiologie, Universität Mainz, Bundesrepublik Deutschland.
出版信息
Z Naturforsch C J Biosci. 1995 May-Jun;50(5-6):419-24.
After incubation with DNA human lymphocytes release neutral and acid DNase activities into the culture medium; the release depends on DNA concentration and time of cultivation. The electrophoretic mobility of the released neutral DNase activity is in accordance with DNase I and the electrophoretic mobility of the released acid DNase activity with DNase II. The released DNase activities do not originate from dead cells and are not influenced by blast cell formation. The anesthetic halothane can inhibit the released neutral and acid DNase activities. Inhalation anesthesia can possibly disturb the correlation between DNA and DNases in human blood.
人淋巴细胞与DNA孵育后，会向培养基中释放中性和酸性DNase活性；这种释放取决于DNA浓度和培养时间。释放的中性DNase活性的电泳迁移率与DNase I一致，释放的酸性DNase活性的电泳迁移率与DNase II一致。释放的DNase活性并非源自死细胞，也不受母细胞形成的影响。麻醉剂氟烷可抑制释放的中性和酸性DNase活性。吸入麻醉可能会干扰人体血液中DNA与DNases之间的相关性。
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