Pruvot F R, Navarro F, Janin A, Labalette M, Masy E, Lecomte-Houcke M, Gambiez L, Copin M C, Dessaint J P
Department of Surgery, Hôpital Calmette, Lille, France.
Transpl Int. 1995;8(4):273-9. doi: 10.1007/BF00346880.
Quantification and localization of the main lymphocyte populations were studied in the livers of normal (n = 8) and brain dead (n = 8) subjects. Cytometric analysis performed on mononuclear cell suspensions obtained from liver biopsies was compared to an automatic image analysis of immunostained sections. The overall number of liver associated lymphocytes was in the usual range of peripheral blood content (2 to 9x10(9) cells). Phenotypic analysis showed predominant NK and CD8+ cells that highly expressed class II antigen and CD25 and CD69 activation markers. Quantitative mapping of these activated lymphocytes revealed their preferential localization in the portal tract and the perisinusoidal area as compared to the pericentrolobular zone, especially in donor livers. This strategic localization could suggest a possible early cooperation between donor lymphocytes and initial infiltrating cells from the recipient and could explain the special immunological status of allografted livers.
对正常受试者（n = 8）和脑死亡受试者（n = 8）的肝脏中主要淋巴细胞群体进行了定量和定位研究。对从肝活检获得的单核细胞悬液进行的细胞计数分析与免疫染色切片的自动图像分析进行了比较。肝脏相关淋巴细胞的总数在周围血含量的正常范围内（2至9×10⁹个细胞）。表型分析显示，主要为NK细胞和CD8⁺细胞，它们高度表达II类抗原以及CD25和CD69激活标志物。这些活化淋巴细胞的定量定位显示，与小叶中心周围区域相比，它们优先定位于门静脉区和窦周区域，在供体肝脏中尤为明显。这种策略性定位可能提示供体淋巴细胞与受体初始浸润细胞之间可能存在早期合作，并可以解释同种异体移植肝脏的特殊免疫状态。
