Fournier-Delpech S, Guérin Y, Magistrini M, Combarnous Y
CNRS-URA 1291, INRA, station de PRMD, Nouzilly, France.
Reprod Nutr Dev. 1995;35(4):427-36.
The motility of ejaculated spermatozoa (% of progressive spermatozoa) has been evaluated visually on 26 adult rams. Half the animals were immunized with a secretory epididymal protein (prealbumin epididymal-specific (PES) ovine) injected according to immunizing protocols (Freund or Hunter adjuvant) intraperitoneally or intradermally (Freund or Hunter adjuvant) or intramuscularly (Hunter adjuvant or PES only). The other animals received the same product without the protein (controls). The product resulted in a strong asthenospermia which parallels the transient presence of anti-PES antibodies in the seminal plasma. A study with 64 ewes showed that the fertility of 10 immunized rams that had recovered sufficient forward motility to ensure fertilization, did not differ from that of control rams.
对26只成年公羊射精精子的活力（进行性运动精子的百分比）进行了肉眼评估。一半的动物按照免疫方案（弗氏或亨特佐剂）通过腹腔内、皮内（弗氏或亨特佐剂）或肌肉内（亨特佐剂或仅注射PES）注射分泌性附睾蛋白（附睾特异性前白蛋白（PES）绵羊）进行免疫。另一半动物接受不含该蛋白的相同产品（对照）。该产品导致严重的弱精子症，这与精浆中抗PES抗体的短暂出现平行。一项对64只母羊的研究表明，10只已恢复足够前向运动能力以确保受精的免疫公羊的生育力与对照公羊没有差异。
