Davies M, Harris S, Rudland P, Barraclough R
Department of Biochemistry, University of Liverpool, UK.
DNA Cell Biol. 1995 Oct;14(10):825-32. doi: 10.1089/dna.1995.14.825.
p9Ka (also known as mts1/18A2/calvasculin/CAPL) is a member of the S-100-related family of small, calcium-binding proteins. Previous studies suggest apparent discrepancies between the expression of the p9Ka gene in rat, mouse, and human tissues. Here we demonstrate that the natural p9Ka gene is expressed at lower levels in mouse than in rat, and that, in mouse but not in rat, p9Ka mRNA is more highly expressed in cells of lymphoid origin. Transgenic mouse strains express rat-p9Ka transgenes in a gene copy-number-dependent manner. The rat p9Ka transgene mRNA shows the same tissue distribution in several lines of transgenic mice, a distribution that is characteristic of the rat, from which the transgenes were derived. These results show that there is a difference in the pattern of expression of the same gene in two closely related species, and that the pattern of expression found in rat is specified by the DNA in the rat gene itself.
p9Ka（也称为mts1/18A2/钙血管素/CAPL）是与S-100相关的小钙结合蛋白家族的成员。先前的研究表明，p9Ka基因在大鼠、小鼠和人类组织中的表达存在明显差异。在此我们证明，天然p9Ka基因在小鼠中的表达水平低于大鼠，并且在小鼠而非大鼠中，p9Ka mRNA在淋巴起源的细胞中表达更高。转基因小鼠品系以基因拷贝数依赖的方式表达大鼠p9Ka转基因。大鼠p9Ka转基因mRNA在多个转基因小鼠品系中显示出相同的组织分布，这种分布是转基因来源的大鼠所特有的。这些结果表明，同一基因在两个密切相关物种中的表达模式存在差异，并且在大鼠中发现的表达模式由大鼠基因本身的DNA所决定。
