Davies M P, Rudland P S, Robertson L, Parry E W, Jolicoeur P, Barraclough R
Department of Biochemistry, University of Liverpool, UK.
Oncogene. 1996 Oct 17;13(8):1631-7.
Increased levels of S100A4 (p9Ka) confer metastatic ability on a normally non-metastatic epithelial cell line. To find out whether S100A4 can induce metastasis in vivo, transgenic mice expressing high levels of S100A4, but which show no phenotypic effect, have been mated with MMTV-neu transgenic mice which succumb to stochastic mammary neoplasia related to expression of the MMTV-neu transgene. Resultant bitransgenic, multiparous, female progeny expressing both S100A4 and Neu have a slightly earlier incidence of palpable mammary tumours than the MMTV-neu offspring and specifically exhibit macroscopic metastatic lesions in the lungs. The S100A4 transgene is expressed in primary and secondary lesions of bitransgenic offspring and its expression is particularly associated with regions of invasion of primary lesions and metastases.
S100A4（p9Ka）水平的升高赋予了一个正常情况下不具有转移能力的上皮细胞系转移能力。为了弄清楚S100A4是否能在体内诱导转移，已将表达高水平S100A4但无表型效应的转基因小鼠与因MMTV-neu转基因表达而易患随机性乳腺肿瘤的MMTV-neu转基因小鼠进行交配。产生的同时表达S100A4和Neu的双转基因、多胎雌性后代出现可触及乳腺肿瘤的发生率略早于MMTV-neu后代，并且特别在肺部出现宏观转移病变。S100A4转基因在双转基因后代的原发性和继发性病变中表达，其表达尤其与原发性病变和转移灶的侵袭区域相关。
