Mixson J M, Spencer P, Moore D L, Chappell R P, Adams S
Department of Restorative Dentistry, University of Missouri-Kansas City, School of Dentistry 64108-2795, USA.
Am J Dent. 1995 Feb;8(1):5-9.
To describe the dentin surface morphology of abrasion/erosion lesions and to chemically characterize in vivo samples of sclerotic dentin.
Baseline polyvinylsiloxane impressions of eight in vivo caries-free lesions were taken. Dentin was collected from retention grooves for FTIR photoacoustic spectroscopic analysis. The cavity preparation was etched for 30 seconds with 37% phosphoric acid, rinsed, and dried. Impressions were taken of the etched surfaces. Epoxy resin dies were made of baseline and etched impressions, sputter-coated and examined at x1000 with the SEM.
Lateral dentin tubule orientation was observed at gingival margins and on occlusal walls. Open cross-sectional tubules were seen at the depth of the groove. The mineral/protein ratio in the FTIR/PAS spectra of in vivo unetched sclerotic dentin samples suggested an increased mineral content.
描述磨损/侵蚀性病变的牙本质表面形态，并对硬化牙本质的体内样本进行化学特征分析。
采集8个体内无龋损病变的基线聚硅氧烷印模。从固位沟收集牙本质用于傅里叶变换红外光声光谱分析。用37%磷酸对窝洞预备进行蚀刻30秒，冲洗并干燥。对蚀刻后的表面取印模。由基线印模和蚀刻印模制作环氧树脂铸型，进行溅射镀膜并在扫描电子显微镜下以1000倍观察。
在牙龈边缘和咬合壁观察到牙本质小管的侧向排列。在沟的深度可见开放的横断面小管。体内未蚀刻的硬化牙本质样本的傅里叶变换红外光谱/光声光谱中的矿物质/蛋白质比率表明矿物质含量增加。
