Sakoolnamarka Rangsima, Burrow Michael F, Prawer Steven, Tyas Martin J
School of Dental Science, The University of Melbourne, Victoria 3010, Australia.
Odontology. 2005 Sep;93(1):35-40. doi: 10.1007/s10266-005-0052-y.
The surface of noncarious cervical lesions (NCCLs) consists of sclerosed dentin. This type of dentin may affect the ability of adhesive restorative materials to bond well to its surface, but little information exists on the chemical nature of this dentin surface and how it may be affected during acidic treatment. The inorganic part of normal dentin and dentin from NCCLs before and after acid conditioning with phosphoric acid or polyacrylic acid was investigated. Ten premolars with NCCLs and four human third molars (control) were used. Replicas of NCCLs were examined using scanning electron microscopy (SEM). Surfaces and longitudinal sections of four NCCLs and control dentin discs were analyzed using Raman spectroscopy. The discs and NCCLs were sectioned, and treated with 35% phosphoric acid or 20% polyacrylic acid/3% aluminum chloride, and Raman spectra obtained. The area under phosphate nu1 of the dentin spectrum was computed to obtain a ratio with the area under the second-order spectrum of a silicon phonon comparative standard. Mean phosphate nu1 and silicon phonon ratios from normal dentin and NCCLs were compared using a linear model with repeated measurements and Tukey's pairwise tests. Mean ratios from different locations of the NCCLs were compared using one-way analysis of variance (ANOVA) and Tukey's pairwise tests. SEM micrographs of NCCL surfaces showed variation from relatively smooth with no dentinal tubule openings to surfaces with occluded tubules. The mean phosphate nu1 and silicon phonon ratios for NCCLs were higher than those of normal dentin in all treatment groups (P < 0.05). Ratios from the untreated specimens were higher than those of the polyacrylic acid-treated specimens, and those for the phosphoric acid-treated group were the lowest (P < 0.05). The ratios obtained for the surfaces of NCCLs were higher than those halfway towards the pulp, and those adjacent to the pulp were the lowest (P < 0.05).
非龋性颈部病变（NCCLs）的表面由硬化牙本质构成。这类牙本质可能会影响黏结性修复材料与它表面良好黏结的能力，但关于这种牙本质表面的化学性质以及在酸处理过程中它可能如何受到影响的信息却很少。研究了正常牙本质以及经磷酸或聚丙烯酸酸蚀处理前后的NCCLs牙本质的无机部分。使用了10颗有NCCLs的前磨牙和4颗人类第三磨牙（对照）。通过扫描电子显微镜（SEM）检查NCCLs的复制品。使用拉曼光谱分析4个NCCLs和对照牙本质片的表面及纵剖面。将牙本质片和NCCLs切片，并用35%磷酸或20%聚丙烯酸/3%氯化铝处理，然后获取拉曼光谱。计算牙本质光谱中磷酸ν1的峰面积，以获得与硅声子对比标准的二阶光谱峰面积的比值。使用具有重复测量的线性模型和Tukey成对检验比较正常牙本质和NCCLs的平均磷酸ν1和硅声子比值。使用单因素方差分析（ANOVA）和Tukey成对检验比较NCCLs不同位置的平均比值。NCCLs表面的SEM显微照片显示出差异，从相对光滑且无牙本质小管开口的表面到小管被堵塞的表面。在所有处理组中，NCCLs的平均磷酸ν1和硅声子比值均高于正常牙本质（P < 0.05）。未处理标本的比值高于聚丙烯酸处理标本的比值，而磷酸处理组的比值最低（P < 0.05）。NCCLs表面获得的比值高于朝向牙髓一半位置的比值，而与牙髓相邻处的比值最低（P < 0.05）。
