[激素对猪细胞系IB-RS-2代谢的影响]

[Effect of hormones on the metabolism of the swine cell line IB-RS-2].

作者信息

Rodrigues M A

出版信息

Arq Inst Biol (Sao Paulo). 1978 Jan-Mar;45(1):37-46.

Abstract

The biological actions of hydrocortisone and insulin on the growth of swine cell line IB-RS-2 were studied. The data obtained showed that hydrocortisone at 2, 4 and 8 x 10(-6) M inhibits cell growth. This effect is directly related to the hormone concentration and to the time of exposure of the cells to the hormone. This inhibitory effect was found to be related to the glucose added to the maintenance medium. On the other hand insulin at 50, 100 and 200 ng/ml increased cell growth and antagonized the inhibitory effect of hydrocortisone. However at 500 ng/ml insulin inhibited cell growth and it was citotoxic.

摘要

研究了氢化可的松和胰岛素对猪细胞系IB-RS-2生长的生物学作用。获得的数据表明，2×10⁻⁶M、4×10⁻⁶M和8×10⁻⁶M的氢化可的松会抑制细胞生长。这种作用与激素浓度以及细胞接触激素的时间直接相关。发现这种抑制作用与添加到维持培养基中的葡萄糖有关。另一方面，50 ng/ml、100 ng/ml和200 ng/ml的胰岛素会促进细胞生长，并拮抗氢化可的松的抑制作用。然而，500 ng/ml的胰岛素会抑制细胞生长且具有细胞毒性。