评估雅培TestPack呼吸道合胞病毒检测试剂盒及一种内部呼吸道合胞病毒酶联免疫吸附测定法用于检测呼吸道吸出物中的呼吸道合胞病毒。

Evaluation of Abbott TestPack RSV and an in-house RSV ELISA for detection of respiratory syncytial virus in respiratory tract aspirates.

作者信息

Obel N, Andersen H K, Jensen I P, Mordhorst C H

机构信息

Department of Virology, Statens Seruminstitut, Copenhagen, Denmark.

出版信息

APMIS. 1995 Jun;103(6):416-8. doi: 10.1111/j.1699-0463.1995.tb01126.x.

DOI:10.1111/j.1699-0463.1995.tb01126.x

PMID:7546643

Abstract

The Abbott TestPack RSV was compared with an in-house RSV enzyme-linked immunosorbent assay (ELISA) for detection of respiratory syncytial virus (RSV) antigen. Nasopharyngeal specimens were obtained from 121 inpatients. RSV antigen was detected in 46 specimens by the Abbott TestPack, 42 of these being confirmed by the in-house RSV ELISA. Of the 75 specimens tested negative in the Abbott TestPack RSV, one was found positive by the in-house RSV ELISA. The sensitivity and specificity of the Abbott TestPack RSV versus the RSV ELISA were 98% and 95% respectively.

摘要

将雅培呼吸道合胞病毒检测试剂盒（Abbott TestPack RSV）与一种内部研发的呼吸道合胞病毒酶联免疫吸附测定法（ELISA）进行比较，以检测呼吸道合胞病毒（RSV）抗原。从121名住院患者中采集了鼻咽标本。通过雅培检测试剂盒在46份标本中检测到RSV抗原，其中42份经内部研发的RSV ELISA法确认。在雅培呼吸道合胞病毒检测试剂盒检测为阴性的75份标本中，有1份经内部研发的RSV ELISA法检测为阳性。雅培呼吸道合胞病毒检测试剂盒相对于RSV ELISA法的灵敏度和特异性分别为98%和95%。