Shimada Y
Department of Neurosurgery, Saitama Medical School, Japan.
No To Shinkei. 1995 Sep;47(9):863-73.
The effects of autogenous omental transposition on spinal cord injury in cats were examined using a cord crush injury model produced by a modification of Allen's weight-dropping technique. Twenty-three cats were divided into two groups: an omental transposition group, and a control group. The animals were sacrificed at 1, 2, 3, and 4 weeks after injury. The injured cords were examined histologically using India ink perfusion, morphometry of the spinal cord cavities and also by somatosensory evoked potential (SEP) studies. After 1 week, hemorrhagic necrosis, cavitation and demyelination of almost the same extent and severity were noted in both groups. These changes diminished with time in the omental transposition group, but tended to persist in the control group. In the omental transposition group, fibrous coats of Goldsmith were newly formed on the injured cord between the incised dura edges. After 3 weeks, the fibrous coat was thickened with active proliferation of fibroblasts and collagen fibers. India ink particles were noticed within the fine vessels of the omentum, fibrous coat and injured cord at 4 weeks. No India ink particles were found in the control group even after 4 weeks. In the somatosensory evoked potentials, biphasic or triphasic waves noted before injury were absent immediately after trauma. At 3 and 4 weeks, 3 of 5 cats with the omentum showed reappearance of the waves, whereas no wave-reappearance was noted in the controls. These results suggest that autogenous omental transposition helps to accelerate the healing process after crush injury to the spinal cord.
采用改良的艾伦重物坠落技术制作脊髓挤压伤模型，研究自体大网膜移植对猫脊髓损伤的影响。23只猫分为两组：大网膜移植组和对照组。在损伤后1、2、3和4周处死动物。使用印度墨汁灌注、脊髓腔形态测量以及体感诱发电位（SEP）研究对损伤的脊髓进行组织学检查。1周后，两组均出现几乎相同程度和严重程度的出血性坏死、空洞形成和脱髓鞘。这些变化在大网膜移植组中随时间减少，但在对照组中倾向于持续存在。在大网膜移植组中，在切开的硬脑膜边缘之间的损伤脊髓上新形成了戈德史密斯纤维包膜。3周后，纤维包膜因成纤维细胞和胶原纤维的活跃增殖而增厚。4周时，在大网膜、纤维包膜和损伤脊髓的微血管内发现印度墨汁颗粒。即使在4周后，对照组中也未发现印度墨汁颗粒。在体感诱发电位方面，损伤前记录的双相或三相波在创伤后立即消失。在3周和4周时，5只接受大网膜移植的猫中有3只出现波的重新出现，而对照组中未观察到波的重新出现。这些结果表明，自体大网膜移植有助于加速脊髓挤压伤后的愈合过程。
