Kisters K, Spieker C, Tepel M, Wenserski F, Zidek W
Medizinische Universitäts-Poliklinik, Münster, Germany.
Magnes Res. 1995 Jun;8(2):169-74.
Plasma magnesium, cytosolic (lymphocytic) and membrane (erythrocytic) magnesium concentrations were determined in 15 controls with normal renal function compared to 12 patients with renal insufficiency (chronic glomerulonephritis, serum creatinine 2.5 +/- 0.8 mg/dl, mean +/- SD). Plasma magnesium concentrations were determined by atomic absorption spectroscopy (AAS), cytosolic free magnesium with the fluorescent indicator Mag-Fura-2, and membrane magnesium by AAS, referred to membrane protein content which was determined according to Bradford's method. Plasma magnesium was 0.91 +/- 0.08 mmol/litre in controls versus 0.95 +/- 0.09 mmol/litre in renal-insufficient patients. Cytosolic free magnesium content was 2.38 +/- 0.75 mmol/litre in controls and 2.61 +/- 0.35 mmol/litre in the renal-insufficient group. In the renal-insufficient group membrane magnesium concentrations were found to be significantly increased as compared to the control group (2.85 +/- 0.62 vs 0.53 +/- 0.22 mmol/g membrane protein, mean +/- SD, P < 0.05). The results show that cell membranes may be of special importance in renal insufficiency in avoiding magnesium overload of the cell and in keeping free cytosolic magnesium stores stable.
对15名肾功能正常的对照组和12名肾功能不全患者（慢性肾小球肾炎，血清肌酐2.5±0.8mg/dl，平均值±标准差）测定了血浆镁、细胞溶质（淋巴细胞）镁和膜（红细胞）镁浓度。血浆镁浓度采用原子吸收光谱法（AAS）测定，细胞溶质游离镁采用荧光指示剂Mag-Fura-2测定，膜镁采用AAS测定，并参照根据Bradford法测定的膜蛋白含量。对照组血浆镁为0.91±0.08mmol/升，肾功能不全患者为0.95±0.09mmol/升。细胞溶质游离镁含量在对照组为2.38±0.75mmol/升，在肾功能不全组为2.61±0.35mmol/升。与对照组相比，肾功能不全组的膜镁浓度显著升高（2.85±0.62对0.53±0.22mmol/g膜蛋白，平均值±标准差，P<0.05）。结果表明，在肾功能不全时，细胞膜对于避免细胞镁过载和维持细胞溶质游离镁储备稳定可能具有特殊重要性。
