Trautinger F, Kindas-Mügge I, Dekrout B, Knobler R M, Metze D
Department of Dermatology, University of Vienna, Austria.
Br J Dermatol. 1995 Aug;133(2):194-202. doi: 10.1111/j.1365-2133.1995.tb02615.x.
The 27-kDa heat shock protein (HSP27) is a member of the small heat shock protein (HSP) family. In addition to its putative function in thermotolerance, this protein may play a part in the regulation of cell growth and differentiation. This study was conducted to assess the significance of the expression of HSP27 in human epidermis and in cutaneous neoplasms. Sixty-two biopsy samples from normal human skin and from inflammatory and neoplastic skin diseases were investigated by immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, using a monoclonal antibody specific for HSP27. In normal human epidermis, HSP27 is expressed in the upper epidermal layers with a cytoplasmic staining pattern. The basal cell layer does not express detectable amounts of HSP27. In hair follicles, staining is mainly confined to the outer root sheath and to the infundibular epithelium. Melanocytes, dermal fibroblasts and endothelial cells do not express detectable amounts of HSP27. HSP27 could not be detected in fetal skin until the 20th week of gestation. Tumour cells in basal and squamous cell carcinomas do not express significant amounts of HSP27. In solar keratoses, seborrhoeic keratoses, human papillomavirus (HPV)-induced hyperproliferative lesions and inflammatory skin conditions, HSP27 expression largely resembles the pattern observed in normal human skin. HSP27 is expressed in a differentiation-related pattern in normal human epidermis and hyperproliferative disorders of the epidermis. We conclude that HSP27 may be regarded as a marker of differentiation in epidermal keratinocytes. Absence of HSP27 in the upper epidermal layers may be a marker for epidermal malignancy.
27 kDa热休克蛋白（HSP27）是小热休克蛋白（HSP）家族的成员。除了其在耐热性方面的假定功能外，该蛋白可能在细胞生长和分化的调节中发挥作用。本研究旨在评估HSP27在人表皮和皮肤肿瘤中的表达意义。使用针对HSP27的单克隆抗体，通过对福尔马林固定石蜡包埋组织切片进行免疫组织化学，研究了62例来自正常人类皮肤以及炎性和肿瘤性皮肤疾病的活检样本。在正常人类表皮中，HSP27在上层表皮中表达，呈细胞质染色模式。基底细胞层不表达可检测量的HSP27。在毛囊中，染色主要局限于外根鞘和漏斗状上皮。黑素细胞、真皮成纤维细胞和内皮细胞不表达可检测量的HSP27。直到妊娠第20周，胎儿皮肤中都检测不到HSP27。基底细胞癌和鳞状细胞癌中的肿瘤细胞不表达大量的HSP27。在日光性角化病、脂溢性角化病、人乳头瘤病毒（HPV）诱导的增生性病变和炎性皮肤疾病中，HSP27的表达在很大程度上类似于在正常人类皮肤中观察到的模式。HSP27在正常人类表皮和表皮增生性疾病中以与分化相关的模式表达。我们得出结论，HSP27可被视为表皮角质形成细胞分化的标志物。表皮上层缺乏HSP27可能是表皮恶性肿瘤的一个标志物。
