Takeuchi T, Tamamoto T, Tamura H, Yamamoto H
Department of Immunology, National Institute of Neuroscience, Tokyo, Japan.
Int Immunol. 1995 Apr;7(4):583-90. doi: 10.1093/intimm/7.4.583.
We previously reported that the nude mouse-derived splenic T cell clone, N-9F, exhibits a proliferative response to the SL10.3 thymic epithelial cell clone. In the present study we generated an Armenian hamster mAb, HS9, specific for SL10.3, which inhibited the N-9F's proliferative response to SL10.3. We performed thymocyte repopulation experiments using fetal liver cells and 2'-deoxyguanosine-treated thymic rudiments. After 14 days of culture, donor fetal liver cells proliferated and differentiated to CD4+CD8+ and CD4-CD8+ with some CD4+CD8- cells in the host thymic rudiments. However, most of the thymocytes remained at a CD4-CD8- immature stage in the presence of HS9 and the cell recovery was reduced to 30% of the control. Immunohistostaining and flow cytometry studies revealed that HS9 reacted with stromal cells of fetal thymus at the earliest from day 14 gestation. Neither thymocytes nor lymph node T cells were stained with HS9. HS9 antigen was distributed not only on thymic subcapsular and cortical stromal cells, but also on peripheral B cells in adult mice. The antigen that HS9 detected was found to be a 50 kDa surface membrane protein on thymic stromal cells. On the other hand, the 50 kDa molecule is associated with two other molecules of 80 and 100 kDa on the B cells. These data indicate that the HS9 antigen may have an important role for early T cell development, especially at a stage from CD4-CD8- to CD4+CD8+, and may have some unknown function on B cells.
我们先前报道，源自裸鼠的脾T细胞克隆N-9F对SL10.3胸腺上皮细胞克隆表现出增殖反应。在本研究中，我们制备了一种针对SL10.3的亚美尼亚仓鼠单克隆抗体HS9，它抑制了N-9F对SL10.3的增殖反应。我们使用胎肝细胞和经2'-脱氧鸟苷处理的胸腺原基进行了胸腺细胞再填充实验。培养14天后，供体胎肝细胞在宿主胸腺原基中增殖并分化为CD4+CD8+和CD4-CD8+细胞，同时还有一些CD4+CD8-细胞。然而，在存在HS9的情况下，大多数胸腺细胞仍处于CD4-CD8-未成熟阶段，细胞回收率降至对照的30%。免疫组织化学染色和流式细胞术研究表明，最早从妊娠第14天起，HS9就与胎儿胸腺的基质细胞发生反应。HS9既不与胸腺细胞也不与淋巴结T细胞染色。HS9抗原不仅分布在成年小鼠胸腺的被膜下和皮质基质细胞上，也分布在外周B细胞上。发现HS9检测到的抗原是胸腺基质细胞上一种50 kDa的表面膜蛋白。另一方面，在B细胞上，50 kDa分子与另外两种80 kDa和100 kDa的分子相关联。这些数据表明，HS9抗原可能对早期T细胞发育具有重要作用，尤其是在从CD4-CD8-到CD4+CD8+的阶段，并且可能在B细胞上具有一些未知功能。
