Takeuchi T, Kuzuhara H, Tamura H, Hiramine C, Hojo K, Yamamoto H
Division of Immunology, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Cell Immunol. 1993 Feb;146(2):324-34. doi: 10.1006/cimm.1993.1030.
A unique 60-kDa surface protein expressed on the thymic epithelial cells was characterized as a potent molecule participating in the interaction between thymic stromal cells (TSC) and immature T cells. Previously, we reported an athymic mouse-derived T cell clone, N-9F, which proliferates on TSC. In the present study, we established a TSC clone, SL10.3, from a BALB/c mouse. SL10.3 has an epithelial cell nature and supports N-9F and fetal thymocytes growth in vitro. The two rat monoclonal antibodies, AS19 and AS32, directed to the SL10.3 cell surface inhibited N-9F and fetal thymocytes growth on SL10.3, suggesting that the reactive molecule may mediate the cellular interaction between TSC and immature T cells. Both antibodies are directed to the same 60-kDa protein with a pI point of 5.4, but to different epitopes on the protein. The 60-kDa protein is distributed on thymic epithelial cells, fibroblasts, and vascular endothelial cells, but not on the hematopoietic cells tested.
在胸腺上皮细胞上表达的一种独特的60 kDa表面蛋白被鉴定为参与胸腺基质细胞（TSC）与未成熟T细胞相互作用的一种有效分子。此前，我们报道了一种源自无胸腺小鼠的T细胞克隆N-9F，它能在TSC上增殖。在本研究中，我们从BALB/c小鼠中建立了一个TSC克隆SL10.3。SL10.3具有上皮细胞性质，并在体外支持N-9F和胎儿胸腺细胞的生长。两种针对SL10.3细胞表面的大鼠单克隆抗体AS19和AS32抑制了N-9F和胎儿胸腺细胞在SL10.3上的生长，这表明该反应性分子可能介导了TSC与未成熟T细胞之间的细胞相互作用。两种抗体都针对同一种等电点为5.4的60 kDa蛋白，但针对该蛋白上不同的表位。这种60 kDa蛋白分布在胸腺上皮细胞、成纤维细胞和血管内皮细胞上，但在所检测的造血细胞上没有分布。
