Besnard P, Foucaud L, Mallordy A, Berges C, Kaikaus R M, Bernard A, Bass N M, Carlier H
Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Université de Bourgogne, Dijon, France.
Biochim Biophys Acta. 1995 Sep 14;1258(2):153-8. doi: 10.1016/0005-2760(95)00114-r.
Expression of the liver fatty acid binding protein (L-FABP) has been studied in the liver of pregnant and lactating rats. The L-FABP concentration found in the cytosol by immuno-enzymatic assay (ELISA) was consistently higher in the dams during the pregnancy and the lactation than in the age-matched virgin females. Paradoxically, a decrease in the L-FABP mRNA level occurred in the maternal liver during the last days of the gestation. This level remained low on days 7 and 14 of the lactation. Since the transcription rate of the L-FABP gene was unchanged in the maternal liver, these data suggest a post-transcriptional regulation of the L-FABP during pregnancy and lactation in the rat. The nutritional adaptations occurring during pregnancy and lactation are not involved in this regulation since a chronic maternal food-restriction failed to correct these modifications. The mechanism of this regulation is presently unknown, but possibilities include hormonally mediated effects.
已对怀孕和哺乳期大鼠的肝脏中肝脂肪酸结合蛋白（L-FABP）的表达进行了研究。通过免疫酶测定法（ELISA）在细胞溶质中发现的L-FABP浓度，在怀孕和哺乳期的母鼠中始终高于年龄匹配的未孕雌性。矛盾的是，在妊娠最后几天，母鼠肝脏中的L-FABP mRNA水平下降。在哺乳期的第7天和第14天，该水平仍然很低。由于母鼠肝脏中L-FABP基因的转录率没有变化，这些数据表明在大鼠怀孕和哺乳期L-FABP存在转录后调控。怀孕和哺乳期发生的营养适应不参与这种调控，因为长期的母体食物限制未能纠正这些变化。这种调控的机制目前尚不清楚，但可能包括激素介导的作用。
