Prows D R, Murphy E J, Schroeder F
Department of Physiology and Pharmacology, Texas A&M University, College Station 77843-4466, USA.
Lipids. 1995 Oct;30(10):907-10. doi: 10.1007/BF02537481.
Differential effects of intestinal (I-FABP) or liver (L-FABP) fatty acid binding proteins on fatty acid uptake and esterification were examined using transfected mouse L-cell fibroblasts. L-FABP, but not I-FABP, expression increased the initial rate and extent of cis-parinaric acid uptake by 50 and 29%, respectively, compared to control cells. I-FABP and L-FABP expression preferentially increased [3H]-oleic acid incorporation into triacylglycerols by 5.5-fold and 3.8-fold, respectively. While both L-FABP and I-FABP increased esterification of [3H]-oleic acid into ethanolamine glycerophospholipids, these proteins had opposite effect on esterification into choline glycerophospholipids. These data show for the first time that distinct FABP differentially affect both fatty acid uptake and intracellular esterification.
利用转染的小鼠L细胞成纤维细胞，研究了肠道脂肪酸结合蛋白（I-FABP）或肝脏脂肪酸结合蛋白（L-FABP）对脂肪酸摄取和酯化的不同影响。与对照细胞相比，L-FABP而非I-FABP的表达分别使顺式-十八碳四烯酸摄取的初始速率和摄取量增加了50%和29%。I-FABP和L-FABP的表达分别使[3H] -油酸掺入三酰甘油的量优先增加了5.5倍和3.8倍。虽然L-FABP和I-FABP都增加了[3H] -油酸酯化生成乙醇胺甘油磷脂，但这些蛋白对酯化生成胆碱甘油磷脂具有相反的作用。这些数据首次表明，不同的脂肪酸结合蛋白对脂肪酸摄取和细胞内酯化有不同影响。
