Lozano M L, Ortuño F, de Arriba F, Rosillo M C, Rivera J, Heras I, Vicente V
Unit of Hematology and Hemotherapy, School of Medicine, Hospital General Universitario, Murcia, Spain.
Ann Hematol. 1995 Sep;71(3):105-10. doi: 10.1007/BF01702644.
Several studies have demonstrated that both CD34+/CD38- and CD34+/HLA-DR- human hematopoietic progenitor cells have properties associated with hematopoietic stem cells. However, the kinetics of these two cell populations in human peripheral blood (PB) after priming with granulocyte colony-stimulating factor (rhG-CSF) has not been investigated. By using flow-cytometric analysis we have shown that administration of rhG-CSF to 14 patients eligible for peripheral blood progenitor cell (PBPC) transplantation led to an increment of CD34+/CD38+ and CD34+/HLA-DR+ cells in the PB that paralleled the increase of total CD34+ cells, indicating that such subpopulations are responsible for the major release of CD34+ cells. Furthermore, rhG-CSF priming led to a significant mobilization of fractions of more immature CD34+/CD38- and CD34+/HLA-DR- cells to the PB. In the leukapheresis preparations, the average frequency of CD34+ cells lacking the CD38 or HLA-DR antigens was low (5% and 30%, respectively), with little overlap between the CD38- and HLA-DR- subpopulations. In addition, the yield of each subset of CD34+ cells (CD34+/CD38 +/- and CD34+/HLA-DR +/-) in the PB correlated with the numbers in the collected material. The results of the present study indicate that administration of rhG-CSF causes a significant increase of CD34+/CD38 +/- and CD34+/HLA-DR +/- cells in PB, and that such cells can be then safely harvested by leukapheresis procedures.
多项研究表明，CD34+/CD38-和CD34+/HLA-DR-人类造血祖细胞均具有与造血干细胞相关的特性。然而，在用粒细胞集落刺激因子（rhG-CSF）启动后，这两种细胞群在人外周血（PB）中的动力学尚未得到研究。通过流式细胞术分析，我们发现，对14例符合外周血祖细胞（PBPC）移植条件的患者给予rhG-CSF后，外周血中CD34+/CD38+和CD34+/HLA-DR+细胞增加，这与总CD34+细胞的增加平行，表明这些亚群是CD34+细胞主要释放的原因。此外，rhG-CSF启动导致更不成熟的CD34+/CD38-和CD34+/HLA-DR-细胞亚群大量动员至外周血。在白细胞分离制剂中，缺乏CD38或HLA-DR抗原的CD34+细胞的平均频率较低（分别为5%和30%），CD38-和HLA-DR-亚群之间几乎没有重叠。此外，外周血中CD34+细胞各亚群（CD34+/CD38+/-和CD34+/HLA-DR+/-）的产量与收集材料中的数量相关。本研究结果表明，给予rhG-CSF可使外周血中CD34+/CD38+/-和CD34+/HLA-DR+/-细胞显著增加，并且这些细胞随后可通过白细胞分离程序安全采集。
