Rusten L S, Jacobsen S E, Kaalhus O, Veiby O P, Funderud S, Smeland E B
Department of Immunology, Norwegian Radium Hospital, Oslo.
Blood. 1994 Sep 1;84(5):1473-81.
Several studies have previously demonstrated enrichment in primitive progenitor cells in subfractions of CD34+ bone marrow (BM) cells not expressing CD38 or HLA-DR (DR) antigens. However, no studies have directly compared these two cell populations with regard to their content of primitive and more committed progenitor cells. Flow cytometric analysis of immunomagnetic isolated CD34+ cells demonstrated little overlap between CD34+CD38- and CD34+DR- progenitor subpopulations in that only 12% to 14% of total CD34+DR- and CD34+CD38- cells were double negative (CD34+CD38-DR-). Although the number of committed myeloid progenitor cells (colony-forming units granulocyte-macrophage) was reduced in both subpopulations, only CD34+CD38- cells were significantly depleted in committed erythroid progenitor cells (burst-forming units-erythroid). In single-cell assay, CD34+CD38- cells showed consistently poorer response to single as opposed to multiple hematopoietic growth factors as compared with unfractionated CD34+ cells, indicating that the CD34+CD38- subset is relatively enriched in primitive hematopoietic progenitor cells. Furthermore, CD34+CD38- and CD34+DR- cells, respectively, formed 3.2-fold and 1.6-fold more high proliferative potential colony-forming cell (HPP-CFC) colonies than did unfractionated CD34+ cells. Finally, CD34+CD38-DR- cells were depleted in HPP-CFCs as compared with CD34+CD38+DR+ cells. The results of the present study suggest that both the CD38- and DR- subfractions of CD34+ bone marrow cells are enriched in primitive hematopoietic progenitor cells, with the CD34+CD38- subpopulation being more highly enriched than CD34+DR- cells.
此前有多项研究表明，在不表达CD38或HLA - DR（DR）抗原的CD34⁺骨髓（BM）细胞亚组分中，原始祖细胞有所富集。然而，尚无研究就原始祖细胞和更定向祖细胞的含量对这两种细胞群体进行直接比较。对免疫磁珠分离的CD34⁺细胞进行流式细胞术分析显示，CD34⁺CD38⁻和CD34⁺DR⁻祖细胞亚群之间几乎没有重叠，因为在总的CD34⁺DR⁻和CD34⁺CD38⁻细胞中，只有12%至14%是双阴性（CD34⁺CD38⁻DR⁻）。尽管两个亚群中定向髓系祖细胞（集落形成单位-粒细胞-巨噬细胞）的数量均减少，但只有CD34⁺CD38⁻细胞中的定向红系祖细胞（爆式红系集落形成单位）显著减少。在单细胞试验中，与未分选的CD34⁺细胞相比，CD34⁺CD38⁻细胞对单一造血生长因子而非多种造血生长因子的反应始终较差，这表明CD34⁺CD38⁻亚群相对富含原始造血祖细胞。此外，CD34⁺CD38⁻和CD34⁺DR⁻细胞分别形成的高增殖潜能集落形成细胞（HPP - CFC）集落比未分选的CD34⁺细胞多3.2倍和1.6倍。最后，与CD34⁺CD38⁺DR⁺细胞相比，CD34⁺CD38⁻DR⁻细胞中的HPP - CFC减少。本研究结果表明，CD34⁺骨髓细胞的CD38⁻和DR⁻亚组分均富含原始造血祖细胞，其中CD34⁺CD38⁻亚群的富集程度高于CD34⁺DR⁻细胞。
