Höffkes H G, Schmidtke G, Schmücker U, Uppenkamp M, Brittinger G
University of Essen, Department of Medicine, Germany.
Ann Hematol. 1995 Sep;71(3):123-8. doi: 10.1007/BF01702647.
Establishing reference ranges by multiparametric immunophenotyping of mature B cells in bone marrow of healthy adults is of interest because the detection of bone marrow infiltration and persistence of light chain restriction, as well as discrimination between reactive and malignant lymphocytes are important applications of B-cell immunophenotyping. To determine the pattern of antigens as expressed by malignant mature B lymphocytes in the present study, bone marrow aspirates of healthy adults were investigated for the presence and percentage frequency of those antigens as defined for immunophenotyping of B cells by the REAL Classification. For this purpose, analysis of CD19-positive B lymphocytes by 'live gate' analysis was performed. The established two-color as well as three-color stainings will serve as a basis for future investigations designed to test multiparametric analysis of B lymphocytes in bone marrow aspirates. All investigated antibodies stained with varying percentage frequency on B-cell subtypes, and no statistical significant difference was found between bone marrow aspirates of women and those of men. On the basis of this analysis, all the reported lineage antigen combinations are present both in malignant B lymphocytes and in normal B cells in considerable percentage frequency. These findings are of importance for follow-up investigations of patients with non-Hodgkin's lymphomas by multiparametric immunophenotyping.
通过对健康成年人骨髓中成熟B细胞进行多参数免疫表型分析来建立参考范围很有意义，因为检测骨髓浸润和轻链限制的持续性，以及区分反应性淋巴细胞和恶性淋巴细胞是B细胞免疫表型分析的重要应用。为了确定本研究中恶性成熟B淋巴细胞所表达的抗原模式，对健康成年人的骨髓穿刺液进行了研究，以确定那些通过REAL分类法定义的用于B细胞免疫表型分析的抗原的存在情况和百分比频率。为此，通过“活门”分析对CD19阳性B淋巴细胞进行了分析。所建立的双色和三色染色将作为未来旨在测试骨髓穿刺液中B淋巴细胞多参数分析的研究的基础。所有研究的抗体在B细胞亚型上的染色频率各不相同，并且在女性和男性的骨髓穿刺液之间未发现统计学上的显著差异。基于此分析，所有报道的谱系抗原组合在恶性B淋巴细胞和正常B细胞中均以相当高的百分比频率存在。这些发现对于通过多参数免疫表型分析对非霍奇金淋巴瘤患者进行随访研究具有重要意义。
