Safer A M
Department of Zoology, Faculty of Science, University of Kuwait.
Eur J Histochem. 1995;39(2):149-56.
Histochemical activity of alkaline phosphatase in the proximal convoluted tubule of the gerbil Meriones crassus has been detected. The reaction product was strongly marked at both the apical surface and the basal part of the cells comprising the basal infoldings with their extended tips and the profiles of the membrane-bound bodies that were easily recognisable by their rounded shape. The reaction product was in the form of fine, uniform deposits in response to cerium chloride as a capture agent. Other cytoplasmic structures did not display these deposits. In addition, the study provided the application of levamisole which caused remarkable reduction of the enzyme activity. Using lead nitrate as a capture agent, the sections showed dense coarse, irregular clusters nearly at the same sites. Control experiments, processed in the absence of the substrate sodium beta-glycerophosphate did not demonstrate the presence of any deposits. The present study, which demonstrates for the first time the presence of alkaline phosphatase activity in the proximal convoluted tubule cells of such desert rodents, does not consider the relationship of the enzyme localization and the basal components of the cells including the nature of the membrane-bound bodies which are still under investigation.
已检测到肥尾沙鼠近曲小管中碱性磷酸酶的组织化学活性。反应产物在构成基底内褶及其延伸尖端的细胞的顶端表面和基部都有强烈标记，以及通过其圆形形状易于识别的膜结合体轮廓。作为捕获剂，反应产物呈细小、均匀沉积物的形式，以应对氯化铈。其他细胞质结构未显示这些沉积物。此外，该研究提供了左旋咪唑的应用，其导致酶活性显著降低。使用硝酸铅作为捕获剂时，切片在几乎相同的部位显示出密集、粗大、不规则的簇状物。在没有底物β-甘油磷酸钠的情况下进行的对照实验未显示有任何沉积物。本研究首次证明了这种沙漠啮齿动物近曲小管细胞中存在碱性磷酸酶活性，但未考虑酶定位与细胞基部成分的关系，包括仍在研究中的膜结合体的性质。
