Nariya H, Kamio Y
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Biosci Biotechnol Biochem. 1995 Aug;59(8):1603-4. doi: 10.1271/bbb.59.1603.
Staphylococcal leukocidin and gamma-hemolysin consist of LukF and LukS for leudocidin and LukF and H gamma II for gamma-hemolysin. To identify the pivotal domain(s) of LukS and H gamma II responsible for their specific cytolytic activities, a series of chimeric genes (lukS/hlg2) were constructed and expressed in Escherichia coli. The results indicate that the C-terminal 24-residue and the N-terminal 57-residue segments of LukS and H gamma II, respectively, are the essential regions for the LukS and H gamma II functions.
葡萄球菌杀白细胞素和γ-溶血素分别由杀白细胞素的LukF和LukS以及γ-溶血素的LukF和HγII组成。为了鉴定LukS和HγII中负责其特定溶细胞活性的关键结构域，构建了一系列嵌合基因（lukS/hlg2）并在大肠杆菌中表达。结果表明，LukS和HγII的C末端24个氨基酸残基片段和N末端57个氨基酸残基片段分别是LukS和HγII功能的必需区域。
