Carlson P, Eerola E, Kontiainen S
Department of Medical Microbiology, Aurora Hospital, Helsinki, Finland.
Zentralbl Bakteriol. 1995 Apr;282(3):232-6. doi: 10.1016/s0934-8840(11)80122-2.
A commercially available biochemical test panel, commercially available diagnostic tablets and gas-liquid chromatography (GLC) of cellular fatty acids were used to find out whether Arcanobacterium haemolyticum and Actinomyces pyogenes could be further differentiated from each other. Xylitol and alpha-methyl-D-glucoside fermentation, Voges-Proskauer reaction and tributyrate hydrolysis were found to be useful additional tests which differentiated Arc. haemolyticum and A. pyogenes. GLC analysis revealed major differences in the cellular 16:0, 18:2(9,12) and 18:1(9) fatty acid composition of the two species. Especially the Voges-Proskauer test available as diagnostic tablets can be easily performed in clinical microbiology laboratories, in addition to the tests now used to differentiate Arc. haemolyticum from A. pyogenes.
使用一种市售的生化检测试剂盒、市售诊断片剂以及细胞脂肪酸的气液色谱法（GLC）来确定溶血隐秘杆菌和化脓放线菌是否能够进一步相互区分。发现木糖醇和α-甲基-D-葡萄糖苷发酵、Voges-Proskauer反应以及三丁酸水解是区分溶血隐秘杆菌和化脓放线菌的有用补充试验。GLC分析揭示了这两个菌种在细胞16:0、18:2(9,12)和18:1(9)脂肪酸组成上的主要差异。特别是作为诊断片剂的Voges-Proskauer试验，除了目前用于区分溶血隐秘杆菌和化脓放线菌的试验外，在临床微生物实验室中也可以轻松进行。
