Bernard K A, Bellefeuille M, Ewan E P
National Laboratory for Bacteriology, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.
J Clin Microbiol. 1991 Jan;29(1):83-9. doi: 10.1128/jcm.29.1.83-89.1991.
Cellular fatty acid (CFA) compositions of 561 asporogenous, aerobic gram-positive rods were analyzed by gas-liquid chromatography as an adjunct to their identification when grown on blood agar at 35 degrees C. The organisms could be divided into two groups. In the first group (branched-chain type), which included coryneform CDC groups A-3, A-4, and A-5; some strains of B-1 and B-3; "Corynebacterium aquaticum"; Brevibacterium liquefaciens; Rothia dentocariosa; and Listeria spp., the rods had sizable quantities of antiesopentadecanoic (Ca15:0) and anteisoheptadecanoic (Ca17:0) acids. Other species with these types of CFA included B. acetylicum, which contained large amounts of isotridecanoic (Ci13:0) and anteisotridecanoic (Ca13:0) acids. CFAs useful for distinguishing among Jonesia denitrificans, Oerskovia spp., some strains of CDC groups B-1 and B-3, Kurthia spp., and Propionibacterium avidum were hexadecanoic (C 16:0) acid, isopentadecanoic (Ci15:0) acid, and Ca15:0). The second group (straight-chained type), which included Actinomyces pyogenes; Arcanobacterium haemolyticum; C. bovis; C. cystitidis; C. diphtheriae; C. flavescens, "C. gentalium"; C. jeikeium; C. kutscheri; C. matruchotii; C .minutissimum; C. mycetoides; C. pilosum; C. pseudodiphtheriticum; "C. pseudogenitalium"; C. pseudotuberculosis; C. renale; CDC groups 1, 2, ANF-1, D-2, E, F-1, F-2, G-1, G-2, and I-2; C. striatum; "C. tuberculostearicum"; C. ulcerans; C. vitarumen; C. xerosis; and Erysipelothrix rhusiopathiae, was typified by significant quantities of hexadecanoic (C16:0) and oleic acids (C18:cis9), with differences in the amounts of linoleic acid (C18:2), stearic acid (C18:0), an unnamed peak (equivalent chain length, 14.966), and small quantities of other known saturated and unsaturated fatty acids. CFA composition of these organisms was sufficiently discriminatory to assist in classification but could not be used as the sole means of identification.
对561株无芽孢需氧革兰氏阳性杆菌的细胞脂肪酸（CFA）组成进行了气液色谱分析，作为在35℃血琼脂上生长时辅助鉴定的手段。这些微生物可分为两组。第一组（支链型）包括棒状杆菌属CDC A - 3、A - 4和A - 5组；B - 1和B - 3组的一些菌株；“水生棒状杆菌”；液化短杆菌；龋齿罗氏菌；以及李斯特菌属，这些杆菌含有大量的反异十五烷酸（Ca15:0）和反异十七烷酸（Ca17:0）。具有这类CFA的其他物种包括乙酰短杆菌，其含有大量的异十三烷酸（Ci13:0）和反异十三烷酸（Ca13:0）。对脱氮琼斯菌、厄氏菌属、CDC B - 1和B - 3组的一些菌株、库特氏菌属和嗜丙酸丙酸杆菌进行区分有用的CFA是十六烷酸（C16:0）、异十五烷酸（Ci15:0）和Ca15:0）。第二组（直链型）包括化脓放线菌；溶血隐秘杆菌；牛棒状杆菌；膀胱炎棒状杆菌；白喉棒状杆菌；微黄棒状杆菌；“生殖棒状杆菌”；杰氏棒状杆菌；库氏棒状杆菌；马氏棒状杆菌；极小棒状杆菌；类真菌棒状杆菌；毛样棒状杆菌；假白喉棒状杆菌；“假生殖棒状杆菌”；假结核棒状杆菌；肾棒状杆菌；CDC 1、2、ANF - 1、D - 2、E、F - 1、F - 2、G - 1、G - 2和I - 2组；纹带棒状杆菌；“结核硬脂酸棒状杆菌”；溃疡棒状杆菌；维氏棒状杆菌；干燥棒状杆菌；以及红斑丹毒丝菌，其典型特征是含有大量的十六烷酸（C16:0）和油酸（C18:cis9），亚油酸（C18:2）、硬脂酸（C18:0）、一个未命名峰（等效链长，14.966）的含量存在差异，以及含有少量其他已知的饱和和不饱和脂肪酸。这些微生物的CFA组成具有足够的鉴别力以辅助分类，但不能用作唯一的鉴定手段。
