Boison J O, Korsrud G O, Papich M G, MacNeil J D
Agriculture & Agri-Food Canada, Food Production and Inspection Branch, Saskatoon, SK.
J AOAC Int. 1995 Sep-Oct;78(5):1144-52.
Four commercially available rapid tests (Brilliant Black reduction test, LacTek test, Charm Farm test, and Charm Test II receptor assay) were compared with a liquid chromatographic (LC) method (lowest quantitatable level of 5 ng/mL) in their efficiency, reliability, and sensitivity to detect penicillin G in bovine plasma. Samples were obtained from 16 steers treated with procaine penicillin G alone or in combination with its long-acting form, benzathine penicillin G. The steers were injected intramuscularly with penicillin G doses ranging from label dose to about 9 times label dose. When results of the Brilliant Black reduction, LacTek, Charm Test II, and Charm Farm tests for penicillin G in plasma (with detection sensitivities of 5, 10, 20, and 30 ng/mL, respectively) were compared with results of LC, none of the rapid tests gave false-positive results. Each rapid test elicited a positive response when used to test bovine plasma containing penicillin G residues at concentrations above the test's detection sensitivity. The simplicity, selectivity, and sensitivity of the rapid tests, coupled with rapidity with which results are obtained, make them suitable for use in large-volume preslaughter screening of penicillin-treated cattle.
将四种市售快速检测方法（亮黑还原试验、LacTek检测、Charm Farm检测和Charm Test II受体分析）与液相色谱（LC）法（最低可定量水平为5 ng/mL）在检测牛血浆中青霉素G的效率、可靠性和灵敏度方面进行了比较。从16头单独使用普鲁卡因青霉素G或与长效型苄星青霉素G联合使用的阉牛身上采集样本。给这些阉牛肌肉注射的青霉素G剂量范围从标签剂量到约9倍标签剂量。当将血浆中青霉素G的亮黑还原试验、LacTek检测、Charm Test II检测和Charm Farm检测结果（检测灵敏度分别为5、10、20和30 ng/mL）与LC结果进行比较时，没有一种快速检测方法出现假阳性结果。当用于检测含有高于检测灵敏度浓度的青霉素G残留的牛血浆时，每种快速检测方法都产生了阳性反应。快速检测方法的简单性、选择性和灵敏度，再加上获得结果的快速性，使其适用于对用青霉素治疗的牛进行大规模宰前筛查。
