Shivaji S, Ahmad M B, Rao K M
Centre for Cellular and Molecular Biology, Hyderabad, India.
Cell Mol Biol (Noisy-le-grand). 1995 Jun;41(4):555-63.
Human endometria were analysed for the synthesis and secretion of proteins following short term culture of human endometrial tissue in the presence of 35S-methionine. During the menstrual cycle secretory proteins of molecular weight (MW) 30, 35, 45, 50, 59, 74, 97 and 135 kDa showed increased synthesis during the proliferative phase. The synthesis of these proteins decreased in the secretory phase but the induction of a 26 kDa protein in the early secretory phase and a 28 kDa protein in the late secretory phase was observed. The synthesis of the above secretory proteins of the human endometrium was also confirmed by two dimensional gel electrophoresis. Further, the results demonstrated that the secretory protein profile of human decidual endometria and endometria exhibiting irregular ripening was identical to that of normal secretory phase endometria. But, endometria exhibiting hyperplasia, cystic glandular hyperplasia and adenomatous glandular hyperplasia presented similar secretory protein profiles which were identical with the secretory protein profile of normal proliferative phase endometrium. The present study confirms that a number of proteins are synthesised by the human endometrium during the normal menstrual cycle and during pregnancy. It also provides data for the first time on the proteins secreted by the endometria exhibiting irregular ripening, hyperplasia, cystic glandular hyperplasia and adenomatous glandular hyperplasia.
在含有³⁵S - 甲硫氨酸的条件下对人子宫内膜组织进行短期培养后，分析人子宫内膜中蛋白质的合成与分泌情况。在月经周期中，分子量（MW）为30、35、45、50、59、74、97和135 kDa的分泌蛋白在增殖期合成增加。这些蛋白质在分泌期合成减少，但在分泌早期观察到一种26 kDa蛋白质的诱导合成，在分泌晚期观察到一种28 kDa蛋白质的诱导合成。人子宫内膜上述分泌蛋白的合成也通过二维凝胶电泳得到证实。此外，结果表明，人蜕膜化子宫内膜和表现出不规则成熟的子宫内膜的分泌蛋白谱与正常分泌期子宫内膜的相同。但是，表现出增生、囊性腺增生和腺瘤性腺增生的子宫内膜呈现出相似的分泌蛋白谱，这些谱与正常增殖期子宫内膜的分泌蛋白谱相同。本研究证实，在正常月经周期和妊娠期间，人子宫内膜会合成多种蛋白质。它还首次提供了关于表现出不规则成熟、增生、囊性腺增生和腺瘤性腺增生的子宫内膜所分泌蛋白质的数据。
