Kilic F, Trevithick J R
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.
Biochem Mol Biol Int. 1995 Apr;35(5):1143-52.
The in vitro effect of glucose on cultured rat lenses was correlated with (i) the time-dependent leakage of lactate dehydrogenase (LDH) into defined medium; (ii) the appearance of the lens under the dissection microscope; and (iii) the leakage of lens cytosolic proteins. A protective effect of 1 mM Vitamin C (VC) was also found: the extent of opacification, LDH and gamma-crystallin release were reduced if 1 mM VC was included in the medium. Using the above parameters provides a new and more rapid technique to follow lens opacification in vitro.
（i）乳酸脱氢酶（LDH）随时间向特定培养基中的泄漏；（ii）在解剖显微镜下晶状体的外观；以及（iii）晶状体胞质蛋白的泄漏。还发现1 mM维生素C（VC）具有保护作用：如果培养基中含有1 mM VC，则浑浊程度、LDH和γ-晶状体蛋白释放均会降低。使用上述参数提供了一种新的、更快速的技术来追踪体外晶状体浑浊情况。
