Kilic F, Trevithick J R
Department of Biochemistry, University of Western Ontario, London, Canada.
Curr Eye Res. 1995 Oct;14(10):943-9. doi: 10.3109/02713689508995134.
The effect of cytochalasin D (CD), an actin monomer-stabilizer, has been studied on cataract development in rat lenses. Cataractogenesis was induced by incubating the rat lenses in medium 199 (M199) containing 10(-5) M CD; by the end of 24 h, lenses first developed a visible opacity. The increased lactate dehydrogenase (LDH) activity in the culture medium, leakage of lens cytosolic proteins into the culture medium and observable development of opacity through a dissection microscope were correlated with cell damage associated with cataract formation. Non-denaturing polyacrylamide gel electrophoresis was used to separate three lens LDH isoenzymes. The effect of 1 mM vitamin C (VC) in reducing LDH leakage was also examined. The protective effect of VC on CD-initiated cataractous lenses is significant. This suggest that a portion of the opacity and lens damage may involve oxidative damage to the membrane-cytoskeleton complex which is started by CD, but partially prevented by VC
肌动蛋白单体稳定剂细胞松弛素D（CD）对大鼠晶状体白内障形成的影响已得到研究。通过将大鼠晶状体在含有10^(-5) M CD的199培养基（M199）中孵育来诱导白内障形成；到24小时结束时，晶状体首先出现可见的混浊。培养基中乳酸脱氢酶（LDH）活性的增加、晶状体胞质蛋白渗漏到培养基中以及通过解剖显微镜观察到的混浊发展与白内障形成相关的细胞损伤有关。使用非变性聚丙烯酰胺凝胶电泳分离三种晶状体LDH同工酶。还研究了1 mM维生素C（VC）在减少LDH渗漏方面的作用。VC对CD引发的白内障晶状体的保护作用显著。这表明部分混浊和晶状体损伤可能涉及由CD引发的对膜 - 细胞骨架复合物的氧化损伤，但可被VC部分阻止
