激光高效液相色谱法测定胸腔积液中的前列腺素

Laser high performance liquid chromatography determination of prostaglandins in pleural effusions.

作者信息

Ikuta N, Sugiyama S, Takagi K, Hayakawa T, Ozawa T

机构信息

Department of Internal Medicine, Faculty of Medicine, University of Nagoya, Japan.

出版信息

Biochem Mol Biol Int. 1995 Jul;36(3):521-7.

PMID:7549950

Abstract

We measured prostaglandins (PGs) in human pleural effusions of 43 patients with various diseases using microcolumn high performance liquid chromatography with a He/Cd laser induced fluorescence detection system. PGD2 was not detected in the transdate effusions. In contrast, PGD2 was detected in pleural exudates (malignant effusions: 0.22 +/- 0.07 nmol/ml, tuberculous effusions: 0.28 +/- 0.08 nmol/ml). PGE2 and 6-keto-PGF1 alpha concentrations in malignant effusions (1.62 +/- 0.17 nmol/ml, 14.40 +/- 1.33 nmol/ml, respectively) were significantly increased compared with tuberculous effusions (0.98 +/- 0.13 nmol/ml, 10.36 +/- 0.92 nmol/ml) and transdates (0.60 +/- 0.06 nmol/ml, 6.91 +/- 0.61 nmol/ml). On the contrary, there was no significant difference in PGF2 alpha concentrations between malignant effusions and tuberculous effusions. From these results, not only PGE2 but also PGD2 and PGI2 might be implicated in the pleural fluid accumulation.

摘要

我们使用配备氦/镉激光诱导荧光检测系统的微柱高效液相色谱法，对43例患有各种疾病的患者的人胸腔积液中的前列腺素（PGs）进行了测量。在漏出液中未检测到PGD2。相比之下，在胸腔渗出液中检测到了PGD2（恶性胸腔积液：0.22±0.07 nmol/ml，结核性胸腔积液：0.28±0.08 nmol/ml）。与结核性胸腔积液（0.98±0.13 nmol/ml，10.36±0.92 nmol/ml）和漏出液（0.60±0.06 nmol/ml，6.91±0.61 nmol/ml）相比，恶性胸腔积液中的PGE2和6-酮-PGF1α浓度（分别为1.62±0.17 nmol/ml，14.40±1.33 nmol/ml）显著升高。相反，恶性胸腔积液和结核性胸腔积液中的PGF2α浓度没有显著差异。从这些结果来看，不仅PGE2，而且PGD2和PGI2可能都与胸腔积液的积聚有关。